![Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Western-Blot-NB100-1137-img0010.jpg "Western Blot: SETDB2 Antibody [NB100-1137] - Staining of Nuclear HeLa lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP, ChIP |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence GEKNGDAKTFWME-C corresponding to N-Terminus according to NP_114121.2, NP_001153780.1. |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_114121.2; NP_001153780.1). |
| Predicted Species | Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SETDB2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Approx 85 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 81.9 kDa according to NP_114121.2). Not suitable on mouse heart and rat heart lysates. This SETDB2 antibody is validated for WB, CHIP, ICC/IF IP from verified customer reviews. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
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WB | Human | 07/18/2018 |
Summary
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reviewed by:
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ChIP | Human | 06/22/2018 |
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reviewed by:
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ICC | Human | 06/18/2018 |
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reviewed by:
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IP | Human | 05/16/2018 |
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reviewed by:
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WB | Human | 01/04/2018 |
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reviewed by:
Verified Customer |
ICC | Human | 02/25/2016 |
Summary
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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| Verified Customer 07/18/2018 |
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| Application: | WB | |
| Species: | Human |
| Verified Customer 06/22/2018 |
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| Application: | ChIP | |
| Species: | Human |
| Verified Customer 06/18/2018 |
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| Application: | ICC | |
| Species: | Human |
| Gene Symbol | SETDB2 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - Human dermal microvascular endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1137-img0007.jpg "Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - Human dermal microvascular endothelial cells were fixed with 4% paraformaldehyde, permeabilized with 0.2% TritonX-100 and immunostained with NB100-1137 (1:100 dilution). Secondary antibody conjugated to Alexafluor 488. Image submitted by a verified customer review.")
![Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Chromatin-Immunoprecipitation-ChIP-NB100-1137-img0009.jpg "Chromatin Immunoprecipitation (ChIP): SETDB2 Antibody [NB100-1137] - Human B-cell nuclear extract (500 ug). Gene of interest promoter primers were used for amplification to detect ChIP efficiency to IgG control. Image submitted by a verified customer review.")
![Western Blot: SETDB2 Antibody [NB100-1137] - RL human non-Hodgkin's lymphoma B cell line whole cell lysate (WCL). Single band around correct MW is specific to the target protein SETDB2. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Western-Blot-NB100-1137-img0005.jpg "Western Blot: SETDB2 Antibody [NB100-1137] - RL human non-Hodgkin's lymphoma B cell line whole cell lysate (WCL). Single band around correct MW is specific to the target protein SETDB2. Image submitted by a verified customer review.")
![Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - SETDB2 in human induced Pluripotent Stem Cells (iPSCs) using anti-SETDB2 antibody. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/SETDB2-Antibody-Chromatin-Immunoprecipitation-NB100-1137-img0006.jpg "Immunocytochemistry/Immunofluorescence: SETDB2 Antibody [NB100-1137] - SETDB2 in human induced Pluripotent Stem Cells (iPSCs) using anti-SETDB2 antibody. Image submitted by a verified customer review.")
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