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SENP2 Antibody (OTI3D3)

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Western Blot: SENP2 Antibody (3D3) [NBP2-03852] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-SENP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; ...read more
Immunocytochemistry/ Immunofluorescence: SENP2 Antibody (3D3) [NBP2-03852] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SENP2.
Immunohistochemistry-Paraffin: SENP2 Antibody (3D3) [NBP2-03852] - Staining of paraffin-embedded Human Kidney tissue using anti-SENP2 mouse monoclonal antibody.
Flow Cytometry: SENP2 Antibody (3D3) [NBP2-03852] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SENP2 antibody, and then analyzed ...read more
Western Blot: SENP2 Antibody (3D3) [NBP2-03852] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: SENP2 Antibody (3D3) [NBP2-03852] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SENP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SENP2 Antibody (3D3) [NBP2-03852] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SENP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SENP2 Antibody (3D3) [NBP2-03852] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SENP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SENP2 Antibody (3D3) [NBP2-03852] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SENP2 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3D3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SENP2 Antibody (OTI3D3) Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 139-523 of human SENP2 (NP_067640) produced in E.coli.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
SENP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SENP2 Antibody (OTI3D3)

  • AXAM2
  • AXAM2Axam2
  • DKFZp762A2316
  • EC 3.4.22
  • EC 3.4.22.68
  • KIAA1331sentrin-specific protease 2
  • SENP2
  • Sentrin/SUMO-specific protease SENP2
  • SMT3IP2
  • SMT3IP2EC 3.4.22.-
  • SMT3-specific isopeptidase 2
  • SUMO1/sentrin/SMT3 specific peptidase 2
  • SUMO1/sentrin/SMT3 specific protease 2

Background

SUMO1 (UBL1; MIM 601912) is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1 into the conjugatable form and catalyze the deconjugation of SUMO1-containing species.[supplied by OMIM]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SENP2
Entrez