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SDHB Antibody (SDHB/6697R) - Azide and BSA Free

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Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827] - Formalin-fixed, paraffin-embedded human paraganglioma stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/6697R).
Immunohistochemistry-Paraffin: SDHB Antibody (SDHB/6697R) - Azide and BSA Free [NBP3-08827] - Formalin-fixed, paraffin-embedded human heart stained with SDHB Recombinant Rabbit Monoclonal Antibody (SDHB/6697R).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
SDHB/6697R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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SDHB Antibody (SDHB/6697R) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07818).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide corresponding to residues in human SDHB was used as an immunogen. (Uniprot: P21912 )
Localization
Cytoplasm, Mitochondria
Marker
Pheochromocytoma Marker
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
SDHB
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for SDHB Antibody (SDHB/6697R) - Azide and BSA Free

  • EC 1.3.5.1
  • FLJ92337
  • IP
  • Iron-sulfur subunit of complex II
  • PGL4
  • SDH
  • SDH1
  • SDH2
  • SDHIP
  • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
  • succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SDHB