Simple Western lane view shows recombinant SARS-CoV-2 Spike S2 Protein, loaded at 50 ng/mL. A specific band was detected for SARS-CoV-2 Spike S2 Protein at approximately 58 kDa (as indicated) using a serial dilution of ...read more
SARS-CoV-2 spike overexpressed 293T cell lysate. Antibody dilution: 1:3000. Two bands for full length spike and cleaved S2 are shown, respectively. Western blot image submitted by a verified customer review.
Western blot shows SARS-CoV-1 Spike (Catalog # 10581-CV), SARS CoV-2 Spike (Catalog # 10586-CV), SArS-CoV-2 Spike S2 Subunit (Catalog # 10584-CV). Membrane was probed with 0.5 ug/mL of SARS Spike Protein Antibody ...read more
Western blot analysis of SARS Spike Protein in (A) transfected and (B) untransfected mouse melanoma cell lysate.
Western blot shows recombinant protein SARS-CoV-2 Spike S2 Subunit. Membrane was probed with 0.5 ug/mL of Rabbit Anti-SARS Spike Protein Polyclonal Antibody (Catalog # NB100-56578) followed by HRP-conjugated Anti-Rabbit ...read more
Simple Western lane view shows SARS-CoV-2 lysate, loaded at 1:20 dilution. SARS-CoV-2 Spike Protein was detected using 6.25 ug/mL of Rabbit Anti-SARS-CoV Spike Protein Polyclonal Antibody (Catalog # NB100-56578) ...read more
The antibody was developed by immunizing rabbits with a synthetic peptide corresponding to amino acids 1124-1140 (C-GNCDVVIGIVNNTVYDP) from the S (Spike glycoprotein)(Spike protein S2') for the Human SARS coronavirus (Genbank accession no. YP_009724390)
Specificity
Full length Spike protein transfected into UM92 cells was used as a positive control and an approximate 139 kDa band was observed. Dot Blot results using recombinant proteins for cross-reactivity testing revealed high reactivity to SARS-CoV-2 Spike Protein, 1000-1200 a.a. (NBP2-90973) and low/no reactivity towards MERS Spike 2 or H1N1 (NBP1-99041).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified
Alternate Names for SARS Spike Protein Antibody
COVID-19 Spike
E2 glycoprotein
E2
Human coronavirus spike glycoprotein
Peplomer protein
S glycoprotein
S
SARS coronavirus S protein
SARS coronavirus Spike Protein
SARS CoV S glycoprotein
SARS CoV Spike protein
SARS CoV
SARS-CoV S protein
SARS-COV Spike
SARSCoV
SARS-COV-2 S protein
SARS-COV-2 Spike glycoprotein
SARSCOV2 Spike protein
Severe acute respiratory syndrome spike glycoprotein
Severe acute respiratory syndrome virus spike glycoprotein
Spike glycoprotein
VGL2
Background
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for SARS Spike Protein Antibody (NB100-56578)(34)
We have publications tested in 2 confirmed species: Human, SARS-CoV-2.
We have publications tested in 6 applications: ICC/IF, IF/IHC, Immunocytochemistry/ Immunofluorescence, Simple Western, WB, Western Blot.
Peacock TP, Brown JC, Zhou J et al. The SARS-CoV-2 variant, Omicron, shows rapid replication in human primary nasal epithelial cultures and efficiently uses the endosomal route of entry. bioRxiv 2022-01-01 (WB)
Zhang Y, Anbir S, McTiernan J et al. Synthesis, Insertion, and Characterization of SARS-CoV-2 Membrane Protein Within Lipid Bilayers bioRxiv 2023-10-02 (WB)
Details: Dilution 1:1000
WB
Vu MN Spike Processing and Protease Usage Effects on SARS-CoV-2 Pathogenesis Thesis 2023-01-01 (WB)
WB
Tan C, Trew J, Peacock T et al. Surveillance of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential bioRxiv 2023-01-20 (Western Blot)
FAQs for SARS Spike Protein Antibody (NB100-56578). (Showing 1 - 4 of 4 FAQs).
Do you know if Sars Spike Protein Antibody (16F1071) will cross react with SARS-2?
NBP2-24746 has no similarity unfortunately. However, NBP2-24808, NBP2-24942, and NB100-56578 have 100% sequence homology with the SARS-CoV-2 Spike Protein.
I am looking for a primary antibody to sars spike protein to the new COVID-19.
NBP1-28850, NB100-56578, NBP2-24808, NBP2-24942 have 100% homology to the new SARS-CoV-2 virus.
Hello can you help me to identify detection antibody for COvid-19 spike protein? NB100-56578 is for spike of the Covid-19 not for the original SARS virus correct?
NB100-56578 was originally developed to the original SARS virus, but the homology of the target sequence of this antibody to the spike protein for COVID-19 is identical, so we can guarantee that it will work for COVID-19 detection.
The spec sheet says that NB100-56578 species cross reactivity 100% wonder what that means…
It means we have blasted homology between our immunogens and COVID19, and this one is 100% homologous. However, we do not have COVID19 samples to validate with, so it is predicted to work on COVID19.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
