We would like an anti-RUNX2 for IHC-P which share cross reactivity with Rat, but not with Human.

Name: RUNX2/CBFA1 Antibody [Unconjugated]
Brand: Novus Biologicals
SKU: AF2006
Rating: 4 (1 reviews)

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Accepted Answer

We don't have any data for our RUNX2 antibodies that confirms they will NOT detect the human protein. When we can confirm that an antibody will not react with a certain species, we display a (-) sign on the datasheet. Otherwise, if the species is not listed it means that it has not been tested.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	HuSpecies Glossary
Applications	WB, ChIP, ICC/IF
Clonality	Polyclonal
Host	Goat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Biotin BAF2006

Catalog# & Formulation	Size	Price
Carrier Free AF2006-SP

Conjugate	Catalog #	Availability	Size	Price
Biotin	BAF2006

RUNX2/CBFA1 Antibody [Unconjugated]

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Publications for RUNX2/CBFA1 Antibody (AF2006)(13)

Review for RUNX2/CBFA1 Antibody (AF2006) (1) 41

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Specificity	Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
Source	N/A
Isotype	IgG
Clonality	Polyclonal
Host	Goat
Gene	RUNX2
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Publications using AF2006	Applications	Species
Polsani, N;Yung, T;Thomas, E;Phung-Rojas, M;Gupta, I;Denker, J;Feng, X;Ibarra, B;Hopyan, S;Atit, RP; Mesenchymal Wnts are required for morphogenetic movements of calvarial osteoblasts during apical expansion bioRxiv : the preprint server for biology 2023-12-07 [PMID: 38106005] (IHC, Transgenic Mouse)	IHC	Transgenic Mouse
Xiaotian Feng, Helen Molteni, Megan Gregory, Jennifer Lanza, Nikaya Polsani, Rachel Wyetzner et al. Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cues bioRxiv 1/19/2023 [PMID: 36711975]
M Minguzzi, V Panichi, S D'Adamo, S Cetrullo, L Cattini, F Flamigni, E Mariani, RM Borzì Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes International Journal of Molecular Sciences, 2021-11-05;22(21):. 2021-11-05 [PMID: 34769441] (Western Blot, Human)	Western Blot	Human
S Somemura, T Kumai, K Yatabe, C Sasaki, H Fujiya, H Niki, K Yudoh Physiologic Mechanical Stress Directly Induces Bone Formation by Activating Glucose Transporter 1 (Glut 1) in Osteoblasts, Inducing Signaling via NAD+-Dependent Deacetylase (Sirtuin 1) and Runt-Related Transcription Factor 2 (Runx2) International Journal of Molecular Sciences, 2021-08-23;22(16):. 2021-08-23 [PMID: 34445787] (Western Blot, Human)	Western Blot	Human
Liangcong Hu, Jing Liu, Hang Xue, Adriana C. Panayi, Xudong Xie, Ze Lin et al. miRNA-92a-3p regulates osteoblast differentiation in patients with concomitant limb fractures and TBI via IBSP/PI3K-AKT inhibition Molecular Therapy - Nucleic Acids 3/5/2021 [PMID: 33717654]
Cheng-Ying Wu, Bin Zhang, Hansol Kim, Stephen K. Anderson, Jeffrey S. Miller, Frank Cichocki Ascorbic Acid Promotes KIR Demethylation during Early NK Cell Differentiation The Journal of Immunology 2020-09-15 [PMID: 32759296]
Sophie A. Millar, Stephen G. John, Christopher W. McIntyre, Vera Ralevic, Susan I. Anderson, Saoirse E. O'Sullivan An Investigation Into the Role of Osteocalcin in Human Arterial Smooth Muscle Cell Calcification Front Endocrinol (Lausanne) 2020-06-10 [PMID: 32587575]
Valentina Sancisi, Gloria Manzotti, Mila Gugnoni, Teresa Rossi, Greta Gandolfi, Giulia Gobbi et al. RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN Nucleic Acids Research 2017-11-02 [PMID: 28981843]
Tao Wu, Tao Shu, Le Kang, Jinhui Wu, Jianzhou Xing, Zhiqin Lu et al. Icaritin, a novel plant-derived osteoinductive agent, enhances the osteogenic differentiation of human bone marrow- and human adipose tissue-derived mesenchymal stem cells International Journal of Molecular Medicine 2017-04-01 [PMID: 28260001]
SHAOFENG AN, XIANGYA HUANG, YAN GAO, JUNQI LING, YIHUA HUANG, YIN XIAO FGF-2 induces the proliferation of human periodontal ligament cells and modulates their osteoblastic phenotype by affecting Runx2 expression in the presence and absence of osteogenic inducers International Journal of Molecular Medicine 2015-09-01 [PMID: 26133673]
Sancisi V, Gandolfi G, Ambrosetti D, Ciarrocchi A Histone Deacetylase Inhibitors Repress Tumoral Expression of the Proinvasive Factor RUNX2. Cancer Res, 2015-03-13;75(9):1868-82. 2015-03-13 [PMID: 25769725] (Western Blot, Human)	Western Blot	Human
McDonald L, Ferrari N, Terry A et al. RUNX2 correlates with subtype-specific breast cancer in a human tissue microarray, and ectopic expression of Runx2 perturbs differentiation in the mouse mammary gland. Dis Model Mech 5/1/2014 [PMID: 24626992]
Olivotto E, Borzi RM, Vitellozzi R, Pagani S, Facchini A, Battistelli M, Penzo M, Li X, Flamigni F, Li J, Falcieri E, Facchini A, Marcu KB Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes. Arthritis Rheum., 2008-01-01;58(1):227-39. 2008-01-01 [PMID: 18163512] (ICC, Human)	ICC	Human
Show All 13 Publications. Collapse Publications.

	Verified Customer 01/06/2015
Application:	WB
Species:	Human

Gene Symbol	RUNX2
Uniprot	Q13950()