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RPL4 Antibody

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Western Blot: RPL4 Antibody [NBP2-20215] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: NCI-H929 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: RPL4 Antibody [NBP2-20215] - Sample (30 ug of whole cell lysate) A: Raji B: K562 C: NCI-H929 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RPL4 Antibody [NBP2-20215] - RPL4 antibody detects RPL4 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: RPL4 ...read more
Immunohistochemistry-Paraffin: RPL4 Antibody [NBP2-20215] - RPL4 antibody detects RPL4 protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. RPL4 antibody ...read more
Immunohistochemistry-Paraffin: RPL4 Antibody [NBP2-20215] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RPL4 Antibody [NBP2-20215] - RPL4 antibody detects RPL4 protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. RPL4 antibody dilution: ...read more

Product Details

Summary
Reactivity Hu, Mu, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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RPL4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human RPL4. The exact sequence is proprietary.
Predicted Species
Bovine (100%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPL4
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for RPL4 Antibody

  • ribosomal protein L4,60S ribosomal protein L1,60S ribosomal protein L4
  • RPL1

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L4E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RPL4
Uniprot