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RNF34 Antibody

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Immunohistochemistry-Paraffin: RNF34 Antibody [NB100-1202] - (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: RNF34 Antibody [NB100-1202] - (4ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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RNF34 Antibody Summary

Immunogen
Peptide with sequence KAGATSMWASCC corresponding to N-Terminus according to NP_079402.2.
Specificity
This antibody is expected to recognize both reported isoforms (NP_919247.1 and NP_079402.2).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RNF34
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 8 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: No signal obtained yet but low background observed in human testis lysate at up to 1 ug/ml. IHC-P: Human kidney shows cytoplasm staining in select DCT.
Control
RNF34 Overexpression Lysate
Publications
Read Publication using NB100-1202.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF34 Antibody

  • CARP1
  • Caspase regulator CARP1
  • Caspases-8 and -10-associated RING finger protein 1
  • E3 ubiquitin-protein ligase RNF34
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ21786
  • FYVE-RING finger protein Momo
  • hRFI
  • Human RING finger homologous to inhibitor of apoptosis protein
  • RFI
  • RIF
  • RIFF
  • ring finger protein 34CARP-1
  • RING finger protein RIFF

Background

The protein encoded by the RNF34 gene contains a RINF finger, a motif known to be involved in protein-protein andprotein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as amodulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alphainduced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have beenreported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RNF34 Antibody (NB100-1202)(1)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF34
Entrez
Uniprot