![Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - RNASEH1 monoclonal antibody (M01), clone 5D10. Analysis of RNASEH1 expression in PC-12.](http://images.novusbio.com/fullsize/RNase-H1-Antibody-5D10-Western-Blot-H00246243-M01-img0007.jpg "Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - RNASEH1 monoclonal antibody (M01), clone 5D10. Analysis of RNASEH1 expression in PC-12.")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 5D10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | RNASEH1 (NP_002927, 189 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AACKAIEQAKTQNINKLVLYTDSMFTINGITNWVQGWKKNGWKTSAGKEVINKEDFVALERLTQGMDIQWMHVPGHSGFIGNEEADRLAREGAKQSED |
| Specificity | RNASEH1 - ribonuclease H1 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RNASEH1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
Secondary Antibodies |
Isotype Controls |
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![Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - RNASEH1 monoclonal antibody (M01), clone 5D10 Analysis of RNASEH1 expression in HeLa.](http://images.novusbio.com/fullsize/RNase-H1-Antibody-5D10-Western-Blot-H00246243-M01-img0006.jpg "Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - RNASEH1 monoclonal antibody (M01), clone 5D10 Analysis of RNASEH1 expression in HeLa.")
![Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - Analysis of RNASEH1 expression in transfected 293T cell line by RNASEH1 monoclonal antibody (M01), clone 5D10.Lane 1: RNASEH1 transfected lysate(32.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/RNase-H1-Antibody-5D10-Western-Blot-H00246243-M01-img0008.jpg "Western Blot: RNase H1 Antibody (5D10) [H00246243-M01] - Analysis of RNASEH1 expression in transfected 293T cell line by RNASEH1 monoclonal antibody (M01), clone 5D10.Lane 1: RNASEH1 transfected lysate(32.1 KDa).Lane 2: Non-transfected lysate.")
![ELISA: RNase H1 Antibody (5D10) [H00246243-M01] - Detection limit for recombinant GST tagged RNASEH1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/RNase-H1-Antibody-5D10-ELISA-H00246243-M01-img0009.jpg "ELISA: RNase H1 Antibody (5D10) [H00246243-M01] - Detection limit for recombinant GST tagged RNASEH1 is approximately 0.03ng/ml as a capture antibody.")
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