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Recombinant Human Ribonuclease A His Protein

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SDS-Page: Recombinant Human Ribonuclease A His Protein [NBP3-12164] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain
SDS-Page: Recombinant Human Ribonuclease A His Protein [NBP3-12164] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Enzyme Activity
Concentration
1 mg/ml

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Recombinant Human Ribonuclease A His Protein Summary

Description
A recombinant protein with a His tag corresponding to the amino acids 29-156 of Human Ribonuclease A

Source: HEK293

Amino Acid Sequence: KESRAKKFQR QHMDSDSSPS SSSTYCNQMM RRRNMTQGRC KPVNTFVHEP LVDVQNVCFQ EKVTCKNGQG NCYKSNSSMH ITDCRLTNGS RYPNCAYRTS PKERHIIVAC EGSPYVPVHF DASVEDST
Details of Functionality
Specific activity is > 5 X 10^4 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.
Source
HEK293
Protein/Peptide Type
Recombinant Protein
Gene
RNASE1
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

Dilutions
  • Enzyme Activity
  • SDS-Page
Theoretical MW
15.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Ribonuclease A His Protein

  • EC 3.1.27.5
  • HP-RNase
  • MGC12408
  • RIB1
  • RIB-1
  • Ribonuclease 1
  • Ribonuclease A
  • ribonuclease pancreatic
  • ribonuclease, RNase A family, 1 (pancreatic)
  • RNase A
  • RNase UpI-1
  • RNS1RNase 1

Background

This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substrate and hydrolyzes 2',3'-cyclic nucleotides, with a pH optimum near 8.0. The encoded protein is monomeric and more commonly acts to degrade ds-RNA over ss-RNA. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RNASE1