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RGS16 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: RGS16 Antibody [NBP2-49076] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry-Paraffin: RGS16 Antibody [NBP2-49076] - Staining of human stomach, upper shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RGS16 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: MCRTLAAFPTTCLERAKEFKTRLGIFLHKSELGCDTGSTGKFEWGSKHSKENRNFSED
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RGS16
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RGS16 Recombinant Protein Antigen (NBP2-49076PEP)

Reactivity Notes

Mouse (81%), Rat (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RGS16 Antibody

  • A28-RGS14
  • A28-RGS14P
  • hRGS-r
  • regulator of G-protein signaling 16
  • regulator of G-protein signalling 16
  • Retinally abundant regulator of G-protein signaling
  • Retinal-specific RGS
  • RGSR
  • RGS-r

Background

RGS16 (regulator of G protein signalling 16) negatively regulates G protein coupled receptor signalling. RGS16 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP bound form. Binds to G(i) alpha and G(o) alpha, but not to G(s) alpha. May play a role in regulating the kinetics of signaling in the phototransduction cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RGS16