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Recombinant Human RENT1/UPF1/hUPF1 GST (N-Term) Protein

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SDS-Page: Recombinant Human RENT1/UPF1/hUPF1 Protein [H00005976-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, MA, PAGE, AP

Order Details

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Recombinant Human RENT1/UPF1/hUPF1 GST (N-Term) Protein Summary

Description
A recombinant protein with a N-terminal GST tag corresponding to the amino acid sequence 1019-1116 of Human RENT1/UPF1/hUPF1

Source: Wheat Germ (in vitro)

Amino Acid Sequence: GRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLS

Preparation
Method
in vitro wheat germ expression system
Details of Functionality
This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
Source
Wheat germ
Protein/Peptide Type
Recombinant Protein
Gene
UPF1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • SDS-Page
  • Western Blot
Theoretical MW
36.52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human RENT1/UPF1/hUPF1 GST (N-Term) Protein

  • ATP-dependent helicase RENT1
  • EC 3.6.1
  • EC 3.6.4.-
  • FLJ43809
  • HUPF1
  • KIAA0221FLJ46894
  • Nonsense mRNA reducing factor 1
  • NORF1
  • NORF1delta helicase
  • pNORF1
  • regulator of nonsense transcripts 1
  • RENT1
  • RENT1UP Frameshift 1
  • UPF1 regulator of nonsense transcripts homolog (yeast)
  • UPF1
  • up-frameshift mutation 1 homolog
  • Up-frameshift suppressor 1 homolog
  • yeast Upf1p homolog

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for RENT1/UPF1/hUPF1 Partial Recombinant Protein (H00005976-Q01) (0)

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Bioinformatics

Gene Symbol UPF1