Recombinant Mouse Thrombopoietin Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1-3). The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10-13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling (15, 16).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), In Vivo, Multiplex Standard.

Showing Publications 1 - 10 of 50. Show All 50 Publications. Collapse Publications.

Publications using 488-TO	Applications	Species
Schirmacher, D;Armagan, Ü;Zhang, Y;Kull, T;Auler, M;Schroeder, T; aiSEGcell: User-friendly deep learning-based segmentation of nuclei in transmitted light images PLoS computational biology 2024-08-01 [PMID: 39178193] (Bioassay, Mouse)	Bioassay	Mouse
Defour, JP;Leroy, E;Dass, S;Balligand, T;Levy, G;Brett, IC;Papadopoulos, N;Mouton, C;Genet, L;Pecquet, C;Staerk, J;Smith, SO;Constantinescu, SN; Constitutive activation and oncogenicity are mediated by loss of helical structure at the cytosolic boundary of thrombopoietin receptor mutant dimers eLife 2023-06-20 [PMID: 37338955] (Bioassay, Mouse)	Bioassay	Mouse
LR Palam, B Ramdas, KM Pickerell, SK Pasupuleti, R Kanumuri, A Cesarano, M Szymanski, BM Selman, UP Davé, G Sandusky, F Perna, S Paczesny, R Kapur Loss of Dnmt3a impairs hematopoietic homeostasis and myeloid cell skewing via the PI3Kinase pathway JCI Insight, 2023-05-08;0(0):. 2023-05-08 [PMID: 36976647] (Bioassay, Mouse)	Bioassay	Mouse
Q Qiu, L Yang, Y Feng, Z Zhu, N Li, L Zheng, Y Sun, C Pan, H Qiu, X Cui, W He, F Wang, Y Yi, M Tang, Z Yang, Y Yang, Z Li, L Chen, Y Hu HDAC I/IIb selective inhibitor Purinostat Mesylate combined with GLS1 inhibition effectively eliminates CML stem cells Bioactive materials, 2022-09-18;21(0):483-498. 2022-09-18 [PMID: 36185739] (Bioassay, Mouse)	Bioassay	Mouse
M Uslu, E Albayrak, F Kocaba? Competitive inhibition of IL-2/IL-2R has a dual effect on HSC ex vivo expansion and IL-2R (CD25) content International immunopharmacology, 2022-07-11;110(0):109035. 2022-07-11 [PMID: 35834953] (Bioassay, Mouse)	Bioassay	Mouse
Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells Nature Communications, 2022-05-12;13(1):2654. 2022-05-12 [PMID: 35551452] (Bioassay, Mouse)	Bioassay	Mouse
A Cardoso, AC Martins, AR Maceiras, W Liu, I Castro, AG Castro, A Bandeira, JP Di Santo, A Cumano, Y Li, P Vieira, M Saraiva Interleukin-10 induces interferon-gamma-dependent emergency myelopoiesis Cell Reports, 2021-10-26;37(4):109887. 2021-10-26 [PMID: 34706233] (Bioassay, Mouse)	Bioassay	Mouse
SR Naidu, M Capitano, J Ropa, S Cooper, X Huang, HE Broxmeyer Chromatin remodeling subunit BRM and valine regulate hematopoietic stem/progenitor cell function and self-renewal via intrinsic and extrinsic effects Leukemia, 2021-10-01;0(0):. 2021-10-01 [PMID: 34599272] (Bioassay, Mouse)	Bioassay	Mouse
K Spinler, J Bajaj, T Ito, B Zimdahl, M Hamilton, A Ahmadi, CS Koechlein, N Lytle, HY Kwon, F Anower-E-K, H Sun, A Blevins, J Weeks, M Kritzik, J Karlseder, MH Ginsberg, PW Park, JD Esko, T Reya A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia Nat Commun, 2020-11-26;11(1):5998. 2020-11-26 [PMID: 33243988] (Cell Culture, Mouse)	Cell Culture	Mouse
NV Bogert, J Furkel, S Din, I Braren, V Eckstein, JA Müller, L Uhlmann, HA Katus, MH Konstandin A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus Sci Rep, 2020-08-13;10(1):13740. 2020-08-13 [PMID: 32792615] (Bioassay, Mouse)	Bioassay	Mouse
L Garcia-Per, M van Eggerm, L van Roon, SA Vloemans, M Cordes, A Schambach, M Rothe, D Berghuis, C Lagresle-P, M Cavazzana, F Zhang, AJ Thrasher, D Salvatori, P Meij, A Villa, JJM Van Dongen, JJ Zwaginga, M van der Bu, HB Gaspar, A Lankester, FJT Staal, K Pike-Overz Successful Preclinical Development of Gene Therapy for Recombinase-Activating Gene-1-Deficient SCID Mol Ther Methods Clin Dev, 2020-03-31;17(0):666-682. 2020-03-31 [PMID: 32322605] (Bioassay, Mouse)	Bioassay	Mouse
YS Lee, MK Kwak, SA Moon, YJ Choi, JE Baek, SY Park, BJ Kim, SH Lee, JM Koh Regulation of bone metabolism by megakaryocytes in a paracrine manner Sci Rep, 2020-02-10;10(1):2277. 2020-02-10 [PMID: 32042021] (Bioassay, Mouse)	Bioassay	Mouse
S Giannini, MM Lee-Sundlo, L Rivadeneyr, CA Di Buduo, R Burns, JT Lau, H Falet, A Balduini, KM Hoffmeiste beta4GALT1 controls beta1 integrin function to govern thrombopoiesis and hematopoietic stem cell homeostasis Nat Commun, 2020-01-17;11(1):356. 2020-01-17 [PMID: 31953383] (Bioassay, Mouse)	Bioassay	Mouse
S Giannini, MM Lee-Sundlo, L Rivadeneyr, CA Di Buduo, R Burns, JT Lau, H Falet, A Balduini, KM Hoffmeiste beta4GALT1 controls beta1 integrin function to govern thrombopoiesis and hematopoietic stem cell homeostasis Nat Commun, 2020;11(1):356. 2020 [PMID: 31953383] (Bioassay, Mouse)	Bioassay	Mouse
JM Bertho, D Kereselidz, L Manens, C Culeux, V Magneron, J Surette, M Blimkie, L Bertrand, H Wyatt, M Souidi, I Dublineau, N Priest, JR Jourdain Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism Sci Rep, 2019-12-27;9(1):19919. 2019-12-27 [PMID: 31882739] (Multiplex Standard, Mouse)	Multiplex Standard	Mouse
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019-09-24;28(13):3329-3337.e5. 2019-09-24 [PMID: 31553904] (Bioassay, Mouse)	Bioassay	Mouse
ML Capitano, N Mor-Vaknin, AK Saha, S Cooper, M Legendre, H Guo, R Contreras-, F Kappes, MA Sartor, CT Lee, X Huang, DM Markovitz, HE Broxmeyer Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling J. Clin. Invest., 2019-05-20;130(0):2555-2570. 2019-05-20 [PMID: 31107242] (Bioassay, Human)	Bioassay	Human
P Wünsche, ESP Eckert, T Holland-Le, A Paruzynski, A Hotz-Wagen, R Fronza, T Rath, I Gil-Farina, M Schmidt, C von Kalle, C Klein, CR Ball, F Herbst, H Glimm Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs Cell Stem Cell, 2018-07-05;23(1):132-146.e9. 2018-07-05 [PMID: 29979988] (Bioassay, Mouse)	Bioassay	Mouse
SK Tasian, JA Casas, D Posocco, S Gandre-Bab, AL Gagne, G Liang, ML Loh, MJ Weiss, DL French, ST Chou Mutation-specific signaling profiles and kinase inhibitor sensitivities of juvenile myelomonocytic leukemia revealed by induced pluripotent stem cells Leukemia, 2018-06-08;0(0):. 2018-06-08 [PMID: 29884903] (Bioassay, Mouse)	Bioassay	Mouse
JP Sasine, HA Himburg, CM Termini, M Roos, E Tran, L Zhao, J Kan, M Li, Y Zhang, SC de Barros, DS Rao, CM Counter, JP Chute Wild-type Kras expands and exhausts hematopoietic stem cells JCI Insight, 2018-06-07;3(11):. 2018-06-07 [PMID: 29875320] (Bioassay, Mouse)	Bioassay	Mouse
HT Fang, CA El Farran, QR Xing, LF Zhang, H Li, B Lim, YH Loh Global H3.3 dynamic deposition defines its bimodal role in cell fate transition Nat Commun, 2018-04-18;9(1):1537. 2018-04-18 [PMID: 29670118] (Bioassay, Mouse)	Bioassay	Mouse
LA Rein, JW Wisler, J Kim, B Theriot, L Huang, T Price, H Yang, M Chen, W Chen, D Sipkins, Y Fedoriw, JK Walker, RT Premont, RJ Lefkowitz ?-Arrestin2 mediates progression of murine primary myelofibrosis JCI Insight, 2017-12-21;2(24):. 2017-12-21 [PMID: 29263312] (Bioassay, Mouse)	Bioassay	Mouse
M Skamagki, C Correia, P Yeung, T Baslan, S Beck, C Zhang, CA Ross, L Dang, Z Liu, S Giunta, TP Chang, J Wang, A Ananthanar, M Bohndorf, B Bosbach, J Adjaye, H Funabiki, J Kim, S Lowe, JJ Collins, CW Lu, H Li, R Zhao, K Kim ZSCAN10 expression corrects the genomic instability of iPSCs from aged donors Nat. Cell Biol., 2017-08-28;19(9):1037-1048. 2017-08-28 [PMID: 28846095] (Differentiation, Differentiation, Mouse)	Differentiation, Differentiation	Mouse
J Huang, A Khan, BC Au, DL Barber, L López-Vásq, NL Prokopishy, M Boutin, M Rothe, JW Rip, M Abaoui, MS Nagree, S Dworski, A Schambach, A Keating, ML West, J Klassen, PV Turner, S Sirrs, CA Rupar, C Auray-Blai, R Foley, JA Medin Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34(+) Cells for Correction of Fabry Disease Mol Ther Methods Clin Dev, 2017-05-12;5(0):241-258. 2017-05-12 [PMID: 28603745] (Bioassay, Mouse)	Bioassay	Mouse
CX Xu, TJ Lee, N Sakurai, K Krchma, F Liu, D Li, T Wang, K Choi ETV2/ER71 regulates hematopoietic regeneration by promoting hematopoietic stem cell proliferation J. Exp. Med., 2017-05-01;0(0):. 2017-05-01 [PMID: 28461595] (Bioassay, Mouse)	Bioassay	Mouse
B Guo, X Huang, S Cooper, HE Broxmeyer Glucocorticoid hormone-induced chromatin remodeling enhances human hematopoietic stem cell homing and engraftment Nat. Med, 2017-03-06;0(0):. 2017-03-06 [PMID: 28263313] (Bioassay, Human)	Bioassay	Human
GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury PLoS ONE, 2017-02-16;12(2):e0171703. 2017-02-16 [PMID: 28207794] (ELISA (Standard), Mouse)	ELISA (Standard)	Mouse
Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors Nat Commun, 2016-11-21;7(0):13396. 2016-11-21 [PMID: 27869129] (Bioassay, Mouse)	Bioassay	Mouse
Modulation of splicing catalysis for therapeutic targeting of leukemia with mutations in genes encoding spliceosomal proteins Nat. Med., 2016-05-02;22(6):672-8. 2016-05-02 [PMID: 27135740] (Bioassay, Mouse)	Bioassay	Mouse
Wang F, Gao J, Malisani A, Xi X, Han W, Wan X Mouse Resistin (mRetn): cloning, expression and purification in Escherichia coli and the potential regulative effects on murine bone marrow hematopoiesis. BMC Biotechnol, 2015-11-16;15(0):105. 2015-11-16 [PMID: 26572487] (Bioassay, Mouse)	Bioassay	Mouse
Schumacher A, Denecke B, Braunschweig T, Stahlschmidt J, Ziegler S, Brandenburg L, Stope M, Martincuks A, Vogt M, Gortz D, Camporeale A, Poli V, Muller-Newen G, Brummendorf T, Ziegler P Angptl4 is upregulated under inflammatory conditions in the bone marrow of mice, expands myeloid progenitors, and accelerates reconstitution of platelets after myelosuppressive therapy. J Hematol Oncol, 2015-06-09;8(0):64. 2015-06-09 [PMID: 26054961] (Bioassay, Mouse)	Bioassay	Mouse
Kim P, Nakano H, Das P, Chen M, Rowe R, Chou S, Ross S, Sakamoto K, Zon L, Schlaeger T, Orkin S, Nakano A, Daley G Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence. J Exp Med, 2015-04-13;212(5):633-48. 2015-04-13 [PMID: 25870201] (Bioassay, Mouse)	Bioassay	Mouse
Begonja A, Pluthero F, Suphamungmee W, Giannini S, Christensen H, Leung R, Lo R, Nakamura F, Lehman W, Plomann M, Hoffmeister K, Kahr W, Hartwig J, Falet H FlnA binding to PACSIN2 F-BAR domain regulates membrane tubulation in megakaryocytes and platelets. Blood, 2015-04-02;126(1):80-8. 2015-04-02 [PMID: 25838348] (Bioassay, Mouse)	Bioassay	Mouse
Bender M, Giannini S, Grozovsky R, Jonsson T, Christensen H, Pluthero F, Ko A, Mullally A, Kahr W, Hoffmeister K, Falet H Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice. Blood, 2014-12-02;125(6):1014-24. 2014-12-02 [PMID: 25468568] (Bioassay, Mouse)	Bioassay	Mouse
Miyawaki K, Arinobu Y, Iwasaki H, Kohno K, Tsuzuki H, Iino T, Shima T, Kikushige Y, Takenaka K, Miyamoto T, Akashi K CD41 marks the initial myelo-erythroid lineage specification in adult mouse hematopoiesis: redefinition of murine common myeloid progenitor. Stem Cells, 2015-03-01;33(3):976-87. 2015-03-01 [PMID: 25446279] (Bioassay, Mouse)	Bioassay	Mouse
Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry C, Challen G, Li W, Wheeler D, Rebel V, Goodell M Dnmt3a loss predisposes murine hematopoietic stem cells to malignant transformation. Blood, 2015-01-22;125(4):629-38. 2015-01-22 [PMID: 25416277] (Bioassay, Mouse)	Bioassay	Mouse
Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function. Nature, 2014-07-27;514(7520):98-101. 2014-07-27 [PMID: 25079320] (Bioassay, Human, Mouse)	Bioassay	Human, Mouse
Weston W, Zayas J, Perez R, George J, Jurecic R Dynamic equilibrium of heterogeneous and interconvertible multipotent hematopoietic cell subsets. Sci Rep, 2014-06-06;4(0):5199. 2014-06-06 [PMID: 24903657] (Bioassay, Mouse)	Bioassay	Mouse
Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, Matsukawa T, Kagiyama Y, Enomoto Y, Kawabata K, Chen-Yi L, Komeno Y, Izawa K, Oki T, Nagae G, Harada Y, Harada H, Otsu M, Aburatani H, Heissig B, Hattori K, Kitamura T Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells. Blood, 2014-05-13;123(25):3932-42. 2014-05-13 [PMID: 24825862] (Bioassay, Mouse)	Bioassay	Mouse
Hannibal R, Chuong E, Rivera-Mulia J, Gilbert D, Valouev A, Baker J Copy number variation is a fundamental aspect of the placental genome. PLoS Genet, 2014-05-01;10(5):e1004290. 2014-05-01 [PMID: 24785991] (Bioassay, Mouse)	Bioassay	Mouse
Park S, Umemoto T, Saito-Adachi M, Shiratsuchi Y, Yamato M, Nakai K Computational promoter modeling identifies the modes of transcriptional regulation in hematopoietic stem cells. PLoS ONE, 2014-04-07;9(4):e93853. 2014-04-07 [PMID: 24710559] (Bioassay, Mouse)	Bioassay	Mouse
Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters. Mol Cell Biol, 2014-03-24;34(11):1976-90. 2014-03-24 [PMID: 24662049] (Bioassay, Mouse)	Bioassay	Mouse
Zimdahl , Bryan, Ito , Takahiro, Blevins , Allen, Bajaj , Jeevisha, Konuma , Takaaki, Weeks , Joi, Koechlein , Claire S, Kwon , Hyog You, Arami , Omead, Rizzieri , David, Broome , H Elizab, Chuah , Charles, Oehler , Vivian G, Sasik , Roman, Hardiman , Gary, Reya , Tannisht Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia. Nat Genet, 2014-02-02;46(3):245-52. 2014-02-02 [PMID: 24487275] (Bioassay, Mouse)	Bioassay	Mouse
Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 2013-11-27;1(6):545-59. 2013-11-27 [PMID: 24371809] (Bioassay, Mouse)	Bioassay	Mouse
Pereira C, Chang B, Qiu J, Niu X, Papatsenko D, Hendry C, Clark N, Nomura-Kitabayashi A, Kovacic J, Ma'ayan A, Schaniel C, Lemischka I, Moore K Induction of a hemogenic program in mouse fibroblasts. Cell Stem Cell, 2013-06-13;13(2):205-18. 2013-06-13 [PMID: 23770078] (Bioassay, Mouse)	Bioassay	Mouse
Cook BD, Liu S, Evans T Smad1 signaling restricts hematopoietic potential after promoting hemangioblast commitment. Blood, 2011-04-22;117(24):6489-97. 2011-04-22 [PMID: 21515822] (Bioassay, Mouse)	Bioassay	Mouse
Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice. Stem Cells, 2008-05-15;26(7):1778-86. 2008-05-15 [PMID: 18483422] (Bioassay, Mouse)	Bioassay	Mouse
Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells. Stem Cells, 2008-03-27;26(6):1628-35. 2008-03-27 [PMID: 18369099] (Bioassay, Human, Mouse)	Bioassay	Human, Mouse
Yilmaz OH, Kiel MJ, Morrison SJ SLAM family markers are conserved among hematopoietic stem cells from old and reconstituted mice and markedly increase their purity. Blood, 2005-10-11;107(3):924-30. 2005-10-11 [PMID: 16219798] (Bioassay, Mouse)	Bioassay	Mouse
Van der Meeren A, Mouthon MA, Vandamme M, Squiban C, Aigueperse J Combinations of cytokines promote survival of mice and limit acute radiation damage in concert with amelioration of vascular damage. Radiat. Res., 2004-05-01;161(5):549-59. 2004-05-01 [PMID: 15161368] (In Vivo, Mouse)	In Vivo	Mouse
Show All 50 Publications. Collapse Publications.

Array 488-TO

• Thrombopoietin/THPO Antibodies
• Thrombopoietin/THPO ELISA Kit
• Thrombopoietin/THPO Lysate
• Thrombopoietin/THPO Protein
• Thrombopoietin/THPO Proteins and Enzymes
• Thrombopoietin/THPO ELISAs