Recombinant Mouse Reelin Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Reelin is a secreted modular glycoprotein that exhibits serine protease activity and is crucial for brain development and function (1-3). It is composed of an N-terminal Reelin domain, eight EGF-like Reelin repeats (RR), and a highly basic C-terminal region (4-6). The N-terminal fragment is suggested to mediate dimerization/oligomerization and receptor recognition, the midpiece receptor binding, and the C-terminal fragment receptor signaling and recognition (1, 5, 7-9). Mouse Reelin is synthesized as a 3461amino acid (aa) precursor protein with a molecular weight of approximately 388 kDa (4). During processing, it can be cleaved between RR2 and RR3 or between RR6 and RR7, producing a 180 kDa and a 330 kDa peptide, respectively (1, 6, 10). Within shared regions in the central fragment, mouse Reelin shares 95% and 97% aa sequence identity with human and rat Reelin, respectively.

Reelin is secreted by Cajal-Retzius cells in the embryo (1, 4, 11). In the adult, it is expressed in the subventricular zone, rostral migratory stream, olfactory bulb, and in the CA1, CA3, and dentate gyrus regions of the hippocampus, as well as in cerebellar granule cells, pyramidal cells of the entorhinal cortex, GABA interneurons, and glial cells (1, 6, 12, 13). Reelin utilizes the receptors VLDLR and ApoE R2, which have been suggested to have divergent roles in Reelin-mediated neuronal migration (1, 2, 6, 12). It has also been shown to interact with Integrin alpha 3 beta 1 and APP (1, 6, 14, 15). During cortical plate development, Reelin controls cell-cell interactions critical for proper neuronal migration and positioning (1, 2, 4, 5, 11, 12, 16). In the adult, it plays a role in dendrite growth and maturation, and synapse formation (2, 6, 15). Additionally, Reelin has been shown to modulate synaptic transmission and plasticity by regulating the subunit composition and conductivity of NMDA receptors (2, 6, 17). Mutation of the human RELN gene results in lissencephaly with cerebellar hypoplasia (11, 18). In addition, abnormal Reelin expression in human brain has been associated with a variety of cognitive pathological conditions including autism, schizophrenia, bipolar disorder, major depression, and Alzheimer’s disease (1, 6, 11, 13, 19, 20). Reelin deficiency found in Reeler mutant mice causes ataxia, tremors, and impaired motor coordination (4, 16). Peripherally, Reelin is important in the development of neuromuscular junctions. But instead of utilizing the locally expressed ApoE R2 and VLDLR, this function requires the serine protease activity of Reelin (3, 21).

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.

Showing Publications 1 - 7 of 7.

Publications using 3820-MR/CF	Applications	Species
RE Niec, T Chu, M Schernthan, S Gur-Cohen, L Hidalgo, HA Pasolli, KA Luckett, Z Wang, SR Bhalla, F Cambuli, RP Kataru, K Ganesh, BJ Mehrara, D Pe'er, E Fuchs Lymphatics act as a signaling hub to regulate intestinal stem cell activity Cell Stem Cell, 2022-06-20;29(7):1067-1082.e18. 2022-06-20 [PMID: 35728595] (Bioassay, Mouse)	Bioassay	Mouse
K Mikulska-R, J Strzelecki, W Nowak Dynamics, nanomechanics and signal transduction in reelin repeats Sci Rep, 2019-12-12;9(1):18974. 2019-12-12 [PMID: 31831824] (Bioassay, Mouse)	Bioassay	Mouse
L Dairaghi, E Flannery, P Giacobini, A Saglam, H Saadi, S Constantin, F Casoni, BW Howell, S Wray Reelin Can Modulate Migration of Olfactory Ensheathing Cells and Gonadotropin Releasing Hormone Neurons via the Canonical Pathway Front Cell Neurosci, 2018-08-03;12(0):228. 2018-08-03 [PMID: 30127721] (Bioassay, Mouse)	Bioassay	Mouse
Hypervulnerability of the adolescent prefrontal cortex to nutritional stress via reelin deficiency Mol. Psychiatry, 2016-11-15;0(0):. 2016-11-15 [PMID: 27843148] (In Vivo, Mouse)	In Vivo	Mouse
Do H, Bruelle C, Tselykh T, Jalonen P, Korhonen L, Lindholm D Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol. J Biol Chem, 2013-08-29;288(41):29613-20. 2013-08-29 [PMID: 23990472] (Bioassay, Rat)	Bioassay	Rat
Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation. J. Cell Biol., 2012-06-04;197(6):837-49. 2012-06-04 [PMID: 22665518] (Bioassay, Mouse)	Bioassay	Mouse
Rebustini IT, Hayashi T, Reynolds AD miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis. Development, 2011-11-24;139(1):191-202. 2011-11-24 [PMID: 22115756] (Bioassay, Mouse)	Bioassay	Mouse

Array 3820-MR/CF

• Reelin Antibodies
• Reelin ELISA Kits
• Reelin Proteins
• Reelin Proteins and Enzymes