Recombinant Mouse EphB4 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin-B2 andephrin-B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

We have publications tested in 6 confirmed species: Human, Mouse, Avian - Quail, Bovine, Chicken, Xenopus.

We have publications tested in 8 applications: Bioassay, Cell Culture, ELISA Capture, Flow Cytometry, ICC, IHC, Immunoprecipitation, In Vivo.

Showing Publications 1 - 10 of 26. Show All 26 Publications. Collapse Publications.

Publications using 446-B4	Applications	Species
Stewen, J;Kruse, K;Godoi-Filip, AT;Zenia, ;Jeong, HW;Adams, S;Berkenfeld, F;Stehling, M;Red-Horse, K;Adams, RH;Pitulescu, ME; Eph-ephrin signaling couples endothelial cell sorting and arterial specification Nature communications 2024-04-03 [PMID: 38570531] (Bioassay, Human)	Bioassay	Human
S Kohara, K Ogawa Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 2022-12-12;10(12):. 2022-12-12 [PMID: 36551990] (Bioassay, Mouse)	Bioassay	Mouse
D Bissen, MK Kracht, F Foss, J Hofmann, A Acker-Palm EphrinB2 and GRIP1 stabilize mushroom spines during denervation-induced homeostatic plasticity Cell Reports, 2021-03-30;34(13):108923. 2021-03-30 [PMID: 33789115] (Bioassay, Mouse)	Bioassay	Mouse
YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo Oxid Med Cell Longev, 2020-06-05;2020(0):1404915. 2020-06-05 [PMID: 32587656] (Bioassay, Mouse)	Bioassay	Mouse
Y Yoon, G Voloudakis, N Doran, E Zhang, C Dimovasili, L Chen, Z Shao, S Darmanis, C Tang, J Tang, VX Wang, PR Hof, NK Robakis, A Georgakopo PS1 FAD mutants decrease ephrinB2-regulated angiogenic functions, ischemia-induced brain neovascularization and neuronal survival Mol. Psychiatry, 2020-06-15;0(0):. 2020-06-15 [PMID: 32541930] (Bioassay, Mouse)	Bioassay	Mouse
W Wang, C Yuan, T Geng, Y Liu, S Zhu, C Zhang, Z Liu, P Wang EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling Stem Cell Res Ther, 2020-01-29;11(1):40. 2020-01-29 [PMID: 31996240] (Cell Culture, Human)	Cell Culture	Human
E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development Elife, 2019-12-23;8(0):. 2019-12-23 [PMID: 31868584] (Bioassay, Mouse)	Bioassay	Mouse
A Grandi, I Zini, S Palese, C Giorgio, M Tognolini, F Marchesani, S Bruno, L Flammini, AM Cantoni, R Castelli, A Lodola, A Fusari, E Barocelli, S Bertoni Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD Front Pharmacol, 2019-06-17;10(0):691. 2019-06-17 [PMID: 31297055] (ELISA Capture, Mouse)	ELISA Capture	Mouse
D Herrero, S Cañón, G Albericio, RM Carmona, S Aguilar, S Mañes, A Bernad Age-related oxidative stress confines damage-responsive Bmi1+ cells to perivascular regions in the murine adult heart Redox Biol, 2019-03-04;22(0):101156. 2019-03-04 [PMID: 30851670] (Bioassay, Flow Cytometry, Mouse)	Bioassay, Flow Cytometry	Mouse
J Yoon, YS Hwang, M Lee, J Sun, HJ Cho, L Knapik, IO Daar TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration Nat Commun, 2018-08-28;9(1):3491. 2018-08-28 [PMID: 30154457] (Bioassay, Xenopus)	Bioassay	Xenopus
JM Baek, YH Cheon, SC Kwak, HY Jun, KH Yoon, MS Lee, JY Kim Claudin 11 regulates bone homeostasis via bidirectional EphB4-EphrinB2 signaling Exp. Mol. Med., 2018-04-27;50(4):50. 2018-04-27 [PMID: 29700355] (Bioassay, Mouse)	Bioassay	Mouse
NA Warren, G Voloudakis, Y Yoon, NK Robakis, A Georgakopo The product of the ?-secretase processing of ephrinB2 regulates VE-cadherin complexes and angiogenesis Cell. Mol. Life Sci., 2018-02-10;0(0):. 2018-02-10 [PMID: 29428965] (Bioassay, Bovine)	Bioassay	Bovine
A Ghori, FB Freimann, M Nieminen-K, I Kremenetsk, K Gertz, M Endres, P Vajkoczy EphrinB2 Activation Enhances Vascular Repair Mechanisms and Reduces Brain Swelling After Mild Cerebral Ischemia Arterioscler. Thromb. Vasc. Biol, 2017-03-02;0(0):. 2017-03-02 [PMID: 28254815] (In Vivo, Mouse)	In Vivo	Mouse
Greenblatt M, Park K, Oh H, Kim J, Shin D, Lee J, Lee J, Singh A, Lee K, Hu D, Xiao C, Charles J, Penninger J, Lotinun S, Baron R, Ghosh S, Shim J CHMP5 controls bone turnover rates by dampening NF-kappaB activity in osteoclasts. J Exp Med, 2015-07-20;212(8):1283-301. 2015-07-20 [PMID: 26195726] (Bioassay, Mouse)	Bioassay	Mouse
Salvucci O, Ohnuki H, Maric D, Hou X, Li X, Yoon S, Segarra M, Eberhart C, Acker-Palmer A, Tosato G EphrinB2 controls vessel pruning through STAT1-JNK3 signalling. Nat Commun, 2015-03-26;6(0):6576. 2015-03-26 [PMID: 25807892] (Bioassay, Human)	Bioassay	Human
White M, Rufaihah A, Liu L, Ghebremariam Y, Ivey K, Cooke J, Srivastava D Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells, 2013-01-01;31(1):92-103. 2013-01-01 [PMID: 23079999] (Flow Cytometry, Human)	Flow Cytometry	Human
Sawamiphak S, Seidel S, Essmann CL, Wilkinson GA, Pitulescu ME, Acker T, Acker-Palmer A Ephrin-B2 regulates VEGFR2 function in developmental and tumour angiogenesis. Nature, 2010-05-05;465(7297):487-91. 2010-05-05 [PMID: 20445540] (Bioassay, ICC, Immunoprecipitation, Mouse)	Bioassay, ICC, Immunoprecipitation	Mouse
Pennisi A, Ling W, Li X, Khan S, Shaughnessy JD, Barlogie B, Yaccoby S The ephrinB2/EphB4 axis is dysregulated in osteoprogenitors from myeloma patients and its activation affects myeloma bone disease and tumor growth. Blood, 2009-07-13;114(9):1803-12. 2009-07-13 [PMID: 19597185] (Bioassay, In Vivo, Human)	Bioassay, In Vivo	Human
Narazaki G, Uosaki H, Teranishi M, Okita K, Kim B, Matsuoka S, Yamanaka S, Yamashita JK Directed and systematic differentiation of cardiovascular cells from mouse induced pluripotent stem cells. Circulation, 2008-07-14;118(5):498-506. 2008-07-14 [PMID: 18625891] (ICC, Mouse)	ICC	Mouse
Masuda J, Usui R, Maru Y Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis. J. Biol. Chem., 2008-02-28;283(19):13148-55. 2008-02-28 [PMID: 18308734] (Bioassay, Human)	Bioassay	Human
Bouzioukh F, Wilkinson GA, Adelmann G, Frotscher M, Stein V, Klein R Tyrosine phosphorylation sites in ephrinB2 are required for hippocampal long-term potentiation but not long-term depression. J. Neurosci., 2007-10-17;27(42):11279-88. 2007-10-17 [PMID: 17942722] (Bioassay, Mouse)	Bioassay	Mouse
Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway. Biochem. Biophys. Res. Commun., 2004-10-08;323(1):17-23. 2004-10-08 [PMID: 15351694] (Bioassay, Human)	Bioassay	Human
Martiny-Baron G, Korff T, Schaffner F, Esser N, Eggstein S, Marme D, Augustin HG Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia, 2004-05-01;6(3):248-57. 2004-05-01 [PMID: 15153337] (Bioassay, Human)	Bioassay	Human
Noren NK, Lu M, Freeman AL, Koolpe M, Pasquale EB Interplay between EphB4 on tumor cells and vascular ephrin-B2 regulates tumor growth. Proc. Natl. Acad. Sci. U.S.A., 2004-04-05;101(15):5583-8. 2004-04-05 [PMID: 15067119] (Bioassay, Human)	Bioassay	Human
le Noble F, Moyon D, Pardanaud L, Yuan L, Djonov V, Matthijsen R, Breant C, Fleury V, Eichmann A Flow regulates arterial-venous differentiation in the chick embryo yolk sac. Development, 2003-12-17;131(2):361-75. 2003-12-17 [PMID: 14681188] (IHC, In Vivo, Avian - Quail, Chicken)	IHC, In Vivo	Avian - Quail, Chicken
Fuller T, Korff T, Kilian A, Dandekar G, Augustin HG Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J. Cell. Sci., 2003-05-06;116(0):2461-70. 2003-05-06 [PMID: 12734395] (Bioassay, ICC, IHC, Human)	Bioassay, ICC, IHC	Human
Show All 26 Publications. Collapse Publications.

Array 446-B4

• EphB4 Antibodies
• EphB4 Lysate
• EphB4 ELISA
• EphB4 Proteins and Enzymes