Recombinant Human VISTA/B7-H5/PD-1H His-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Platelet receptor Gi24, also known as Dies1, VISTA, SISP1 and B7-H5, is a 55-65 kDa transmembrane glycoprotein with homology to B7-like immune costimulatory molecules (1, 2). Mature human Gi24 contains a 162 amino acid (aa) extracellular domain (ECD) with one V-type Ig-like domain, a 21 aa transmembrane segment, and a 96 aa cytoplasmic domain. Within the ECD, human Gi24 shares 70% and 67% aa sequence identity with mouse and rat Gi24, respectively (3). The 30 kDa ECD can be shed by MT1-MMP, with a 25-30 kDa fragment remaining in the membrane (3). Gi24 promotes both MT1-MMP expression and the MT1-MMP mediated activation of MMP-2 (3). Gi24 supports the differentiation of embryonic stem cells (ESC) and enhances BMP-4 induced signaling in ESC, but is also down-regulated following BMP-4 exposure (4, 5). It binds to BMP-4 directly, and also associates with the type I BMP receptor Activin RIB/ALK-4 (4, 5). Gi24 is expressed on the surface of naïve CD4⁺ T cells, regulatory T cells, and adipocytes (6, 7). It is up-regulated in vivo on activated monocytes and dendritic cells (5). Gi24 inhibits CD4⁺ and CD8⁺ T cell proliferation, and their production of IL-2 and IFN-gamma (6). Its expression on tumor cells attenuates the anti-tumor immune response and enables more rapid tumor progression (6). In contrast, Gi24 limits disease progression in the autoimmune disease model EAE (6). Gi24 also acts as a co-inhibitory receptor on alloreactive T cells to regulate T cell tolerance (8).

Array 9057-B7

• VISTA/B7-H5/PD-1H Antibodies
• VISTA/B7-H5/PD-1H Lysate
• VISTA/B7-H5/PD-1H Proteins
• VISTA/B7-H5/PD-1H Proteins and Enzymes
• VISTA/B7-H5/PD-1H ELISA