Recombinant Human SIRP alpha/CD172a His-tag Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Signal regulatory protein alpha (SIRP alpha, designated CD172a), also called SHPS-1 (SHP substrate 1) and previously, MyD-1 (Myeloid/Dendritic-1), is a monomeric ~90 kDa type I transmembrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin superfamily (1-4). SIRPs are paired receptors, with similar extracellular domains but differing C-termini and functions (1, 2). The 503 amino acid (aa) human SIRP alpha contains a 342 aa extracellular domain (ECD), with one V-type, and two C1 type Ig domains, and three potential N glycosylation sites. It has a 110 aa cytoplasmic sequence with ITIM motifs that recruit tyrosine phosphatases SHP-1 and SHP-2 when phosphorylated (4). Human SIRP alpha has more than 40 described polymorphisms, including the prominent BIT (Brain Ig like molecule with Tyrosine-based activation motifs, also called SIRP alpha 2 or PTPNS) (5). One reported isoform lacks aa 1-101, which eliminates most of the V type Ig domain. Human SIRP alpha ECD shares 61%, 60%, 71%, 72% and 73% aa identity with mouse, rat, porcine, bovine and equine SIRP alpha, respectively; it shares 84% and 76% aa identity with human SIRP beta 1 and SIRP gamma, respectively (2). SIRP alpha is expressed mainly on myeloid cells, including macrophages, neutrophils, dendritic and Langerhans cells (3-6). It is also found on neurons, smooth muscle and endothelial cells (7-9). SIRP alpha shows adhesion to the ubiquitous CD47/IAP (integrin associated protein), while SIRP gamma binds more weakly and SIRP alpha 1 does not bind at all (1, 2). Mouse and human SIRP alpha -CD47 binding only cross-reacts for specific polymorphisms and influences engraftment of xenotransplanted stem cells (6, 10). SIRP alpha engagement generally produces a negative regulatory signal (4). Low SIRP alpha recognition of CD47, which occurs on aged erythrocytes or platelets or xenogenic cells, promotes clearance of CD47low cells from circulation (11, 13). SIRP alpha recognition of surfactants SP-A and SP-D in the lung can inhibit alveolar macrophage cytokine production (14). The CD47 integrin-SIRP alpha interaction is reported to promote macrophage fusion during osteoclastogenesis (15).

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, ELISA Standard.

Showing Publications 1 - 2 of 2.

Publications using 9378-SA	Applications	Species
YV Vladimirov, MK Mølmer, KW Antonsen, N Møller, N Rittig, MC Nielsen, HJ Møller A New Serum Macrophage Checkpoint Biomarker for Innate Immunotherapy: Soluble Signal-Regulatory Protein Alpha (sSIRPalpha) Biomolecules, 2022-07-04;12(7):. 2022-07-04 [PMID: 35883493] (ELISA Standard, Human)	ELISA Standard	Human
PE van Bommel, Y He, I Schepel, MAJM Hendriks, VR Wiersma, RJ van Ginkel, T van Meerte, E Ammatuna, G Huls, DF Samplonius, W Helfrich, E Bremer CD20-selective inhibition of CD47-SIRP? don't eat me signaling with a bispecific antibody-derivative enhances the anticancer activity of daratumumab, alemtuzumab and obinutuzumab Oncoimmunology, 2017-10-31;7(2):e1386361. 2017-10-31 [PMID: 29308308] (Bioassay, Human)	Bioassay	Human

Array 9378-SA

• SIRP alpha/CD172a Antibodies
• SIRP alpha/CD172a Lysates
• SIRP alpha/CD172a Proteins
• SIRP alpha/CD172a ELISA
• SIRP alpha/CD172a Proteins and Enzymes