Reactivity | HuSpecies Glossary |
Applications | Bioactivity |
Format | Carrier-Free |
Details of Functionality | Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. Kiyozumi, D. et al. (2005) Exp. Cell Res. 306:9. When 5 x 104 cells/well are added to rhQBRICK/Fc Chimera coated plates (2.5 µg/mL, 100 µL/well), approximately 60%‑80% will adhere after 1 hour at 37° C. Optimal dilutions should be determined by each laboratory for each application. |
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Source | Chinese Hamster Ovary cell line, CHO-derived human QBRICK/FREM1 protein
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Accession # | |||||||
N-terminal Sequence | Ser22 |
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Structure / Form | Oligomer |
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Protein/Peptide Type | Recombinant Proteins |
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Gene | FREM1 |
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Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
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Endotoxin Note | <1.0 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 67.8 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE | 75-85 kDa, reducing conditions |
Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl. |
Purity | >90%, by SDS-PAGE under reducing conditions and visualized by silver stain |
Reconstitution Instructions | Reconstitute at 100 μg/mL in sterile PBS. |
QBRICK, also known as Frem1 (Fras1-related extracellular matrix gene1) is a 244 kDa (predicted), secreted, extracellular matrix glycoprotein and member of the Fras1 family of proteins (1 - 3). Human QBRICK is synthesized as a 2179 amino acid (aa) precursor that has a 21 aa signal sequence and a 2158 aa mature chain (SwissProt #: Q5H8C1). The mature chain is made up of an N-terminal variable region domain containing an Arg-Gly-Asp (RGD) cell adhesive motif, 12 consecutive chondroitin sulfate proteoglycan (CSPG) repeats of approximately 120 aa, a Calx-beta domain, a second RGD sequence, and a C-terminal C-type lectin-like domain, respectively (1). In addition, there are five potential sites of N-linked glycosylation. Multiple splicing variants produce four isoforms for human QBRICK. Because of the characteristic feature of the 12 CSPG repeats, the protein was named QBRICK: “Q” stands for queen and is taken from the queen being the twelfth in a suit of playing cards, and “BRICK” stands for the repeating unit (1). Human QBRICK shares 78% aa sequence identity with mouse QBRICK. QBRICK is localized to the basement membrane in mesenchymal tissue (3). QBRICK plays a role in epidermal differentiation and is required for epidermal adhesion during embryonic development. QBRICK mediates cell-substratum adhesion through alpha V or alpha 8 integrins (1 - 4). Mutations in QBRICK and other Fras1 family proteins (i.e. Fras1 and Frem2) are associated with Fraser syndrome, a recessive multiorgan disorder characterized by crypthophthalmos, syndactyly, renal agenesis, and a variety of morphogenetic defects (2 - 4). It is postulated that QBRICK, Fras1 and Frem2 make up a ternary complex that act together to ensure adhesion between the epidermal basement membrane and the underlying mesenchyme in embryonic skin (3).
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