Recombinant Human Netrin-1 Protein

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Human Netrin-1 (netr: Sanskrit for “one who guides”) is a 75 kDa glycoprotein that is closely related to the laminin  gamma  domain and functions as a chemoattractive or chemorepulsive guidance cue in the central nervous system (CNS) during development (1, 2). The protein is synthesized as a 604 amino acid (aa) precursor that contains a 24 aa signal sequence and a 580 aa mature chain. Residues 46-283 constitute a laminin N-terminal domain (domain VI), while 285-453 make up three laminin-type epidermal growth factor‑like domains. There is a final domain that runs from aa 487-601 that qualifies as a Netrin-1 like domain. It is also known as domain C in the context of C. elegans. There are four potential sites for N‑linked glycosylation. Human Netrin-1 is 99% aa identical to mouse and rat Netrin-1. Netrin-1 is expressed in adult and embryonic tissues. In the adult, the protein is expressed in Schwann cells, oligodendrocytes and multiple neurons. In embryonic tissues, Netrin-1 is found in somatic mesoderm, heart, branchial pouch, and neuroepithelium. Netrin-1 is a secreted protein that, in addition to its involvement in outgrowth and migration orientation in the developing CNS, plays a significant role in the morphogenesis of endothelial cells and vascular smooth-muscle cells. It is also involved in the processes of cytoskeleton reorganization, angiogenesis, epithelial cell adhesion, and cell migration in the lungs, mammary gland, and pancreas (1, 3).

Netrin-1 effects are controlled through different transmembrane receptors (2). Four of the receptors exist in the Unc-5 (Unc=uncontrolled behaviorally) family of proteins, and these include Unc5h1, Unc5h2, Unc5h3/RCM, and Unc5h4. There are also two receptors that belong to the UNC-40 family of molecules. The first is DCC (deleted in colorectal cancer), and the second is neogenin (newly‑generated). UNC-5 receptors are noted to mediate the attraction response of axons and netrins. However, UNC-5 molecules, when co‑expressed with DCC in the presence of Netrin-1, will mediate repulsion by varying cellular calcium levels (3). The adenosine A2b receptor may also be involved in chemoattraction, either by binding directly with Netrin-1 or by serving as a co‑receptor for DCC. These receptors are known as dependence receptors because they depend on their ligand, in this case Netrin-1, for survival (4). Unbound, the receptors induce a specific death signal (4). It is the dysregulation of these receptor systems that may have important roles in tumor biology. DCC and UNC5 proteins make up a system for either initiating or inhibiting apoptosis (4), and it is now believed that Netrin-1 and its dependence receptors play a major role in tumor biology (4-6).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, In Vivo, Stimulation.

Showing Publications 1 - 10 of 18. Show All 18 Publications. Collapse Publications.

Publications using 6419-N1	Applications	Species
Perampalam, P;MacDonald, JI;Zakirova, K;Passos, DT;Wasif, S;Ramos-Valdes, Y;Hervieu, M;Mehlen, P;Rottapel, R;Gibert, B;Correa, RJM;Shepherd, TG;Dick, FA; Netrin signaling mediates survival of dormant epithelial ovarian cancer cells eLife 2024-07-18 [PMID: 39023520] (Bioassay, Human)	Bioassay	Human
G Untiveros, A Raskind, L Linares, A Dotti, L Strizzi Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive Melanoma Cells International Journal of Molecular Sciences, 2022-10-22;23(21):. 2022-10-22 [PMID: 36361539] (Bioassay, Human)	Bioassay	Human
A Mentxaka, J Gómez-Ambr, B Ramírez, A Rodríguez, S Becerril, G Neira, V Valentí, R Moncada, C Silva, X Unamuno, JA Cienfuegos, J Escalada, G Frühbeck, V Catalán Netrin-1 Promotes Visceral Adipose Tissue Inflammation in Obesity and Is Associated with Insulin Resistance Nutrients, 2022-10-18;14(20):. 2022-10-18 [PMID: 36297056] (Stimulation, Human)	Stimulation	Human
J Ablain, A Al Mahi, H Rothschild, M Prasad, S Aires, S Yang, ME Dokukin, S Xu, M Dang, I Sokolov, CG Lian, LI Zon Loss of NECTIN1 triggers melanoma dissemination upon local IGF1 depletion Nature Genetics, 2022-10-13;0(0):. 2022-10-13 [PMID: 36229674] (Bioassay, Human)	Bioassay	Human
BS Casas, G Vitória, CP Prieto, M Casas, C Chacón, M Uhrig, F Ezquer, M Ezquer, SK Rehen, V Palma Schizophrenia-derived hiPSC brain microvascular endothelial-like cells show impairments in angiogenesis and blood-brain barrier function Molecular Psychiatry, 2022-06-15;0(0):. 2022-06-15 [PMID: 35705634] (Bioassay, Human)	Bioassay	Human
JT Chen, L Schmidt, C Schürger, MK Hankir, SM Krug, HL Rittner Netrin-1 as a Multitarget Barrier Stabilizer in the Peripheral Nerve after Injury International Journal of Molecular Sciences, 2021-09-18;22(18):. 2021-09-18 [PMID: 34576252] (In Vivo, Rat)	In Vivo	Rat
A Abdullah, C Herdenberg, H Hedman Netrin-1 functions as a suppressor of bone morphogenetic protein (BMP) signaling Scientific Reports, 2021-04-21;11(1):8585. 2021-04-21 [PMID: 33883596] (Bioassay, Human)	Bioassay	Human
B Mondal, H Jin, S Kallappago, Y Sedkov, T Martinez, MF Sentmanat, GJ Poet, C Li, Y Fan, SM Pruett-Mil, HM Herz The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth Elife, 2020-04-16;9(0):. 2020-04-16 [PMID: 32297854] (Bioassay, Mouse)	Bioassay	Mouse
CP Prieto, BS Casas, P Falcón, A Villanueva, P Lois, J Lattus, V Palma Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus Int J Mol Sci, 2019-03-20;20(6):. 2019-03-20 [PMID: 30897795] (Bioassay, Human)	Bioassay	Human
J Chen, Y Xuan, Y Chen, T Wu, L Chen, H Guan, S Yang, J He, D Shi, Y Wang Netrin-1 alleviates subarachnoid haemorrhage-induced brain injury via the PPAR?/NF-KB signalling pathway J. Cell. Mol. Med., 2019-01-07;0(0):. 2019-01-07 [PMID: 30614619] (In Vivo, Mouse)	In Vivo	Mouse
F Huang, M Ben Aissa, G Lévesque, M Carreau FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis BMC Res Notes, 2018-09-12;11(1):662. 2018-09-12 [PMID: 30213274] (Bioassay, Human)	Bioassay	Human
I Hernandez-, S Koss, S Sharma, MJ Pitman Influence of Netrin-1 on Reinnervation of Laryngeal Muscles Following Recurrent Laryngeal Nerve Injury Neurosci. Lett., 2017-05-17;0(0):. 2017-05-17 [PMID: 28527715] (In Vivo, Rat)	In Vivo	Rat
Q Shao, T Yang, H Huang, F Alarmanazi, G Liu Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion J. Neurosci., 2017-05-08;0(0):. 2017-05-08 [PMID: 28483977] (Bioassay, Human)	Bioassay	Human
Z Xie, L Huang, B Enkhjargal, C Reis, W Wan, J Tang, Y Cheng, JH Zhang Intranasal administration of recombinant Netrin-1 attenuates neuronal apoptosis by activating DCC/APPL-1/AKT signaling pathway after subarachnoid hemorrhage in rats Neuropharmacology, 2017-03-24;0(0):. 2017-03-24 [PMID: 28347836] (In Vivo, Rat)	In Vivo	Rat
Deming P, Campbell S, Stone J, Rivard R, Mercier A, Howe A Anchoring of protein kinase A by ERM (ezrin-radixin-moesin) proteins is required for proper netrin signaling through DCC (deleted in colorectal cancer). J Biol Chem, 2015-01-09;290(9):5783-96. 2015-01-09 [PMID: 25575591] (Bioassay, Human)	Bioassay	Human
Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation. J Bone Miner Res, 2015-05-01;30(5):837-54. 2015-05-01 [PMID: 25483983] (Bioassay, Human)	Bioassay	Human
Huang F, Ben Aissa M, Magron A, Huard C, Godin C, Levesque G, Carreau M The Fanconi anemia group C protein interacts with uncoordinated 5A and delays apoptosis. PLoS ONE, 2014-03-27;9(3):e92811. 2014-03-27 [PMID: 24676280] (Bioassay, Human)	Bioassay	Human
Shimizu A, Nakayama H, Wang P, Konig C, Akino T, Sandlund J, Coma S, Italiano J, Mammoto A, Bielenberg D, Klagsbrun M Netrin-1 promotes glioblastoma cell invasiveness and angiogenesis by multiple pathways including activation of RhoA, cathepsin B, and cAMP-response element-binding protein. J Biol Chem, 2012-11-29;288(4):2210-22. 2012-11-29 [PMID: 23195957] (Bioassay, Human)	Bioassay	Human
Show All 18 Publications. Collapse Publications.

Array 6419-N1

• Netrin-1 Antibodies
• Netrin-1 ELISA Kit
• Netrin-1 Protein
• Netrin-1 Proteins and Enzymes