UNC5H2/UNC5B Antibody [Unconjugated]

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,939,271, 6,277,585, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues (including rat UNC5H1 and H2, mouse UNC5H2 and H3 (also known as rostral cerebellar malformation, RCM), and human UNC5H3 and H4) are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2).

UNC5 family proteins are receptors for the netrin/UNC6 (netr: Sanskrit for “one who guides”) family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors that share sequence similarity with proteins of the NCAM family, and adenosine A2b receptor, a G protein-coupled seven‑transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).

Rat UNC5H2 cDNA encodes a 945 amino acid residues (aa) type I membrane protein with a putative 26 aa signal peptide and 347 aa extracellular domain. The extracellular domain of rat UNC5H2 shares approximately 65%, 73% and 73% aa sequence similarity with that of rat UNC5H1, human UNC5H3 and mouse UNC5H3, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Cell Migration, Functional Assay, ICC, IHC-Fr, IHC-P, Immunoprecipitation, Neutralization, Western Blot.

Showing Publications 1 - 10 of 16. Show All 16 Publications. Collapse Publications.

Publications using AF1006	Applications	Species
W Xiao, A Pinilla-Ba, J Faulkner, X Song, P Prabhakar, H Qiu, KW Moremen, A Ludwig, PJ Dempsey, P Azadi, L Wang Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis Scientific Reports, 2022-03-14;12(1):4352. 2022-03-14 [PMID: 35288626] (Cell Migration, Neutralization, Western Blot, Human, Mouse)	Cell Migration, Neutralization, Western Blot	Human, Mouse
K Boyé, LH Geraldo, J Furtado, L Pibouin-Fr, M Poulet, D Kim, B Nelson, Y Xu, L Jacob, N Maissa, D Agalliu, L Claesson-W, SL Ackerman, A Eichmann Endothelial Unc5B controls blood-brain barrier integrity Nature Communications, 2022-03-04;13(1):1169. 2022-03-04 [PMID: 35246514] (Immunoprecipitation, Mouse)	Immunoprecipitation	Mouse
Sonia Taïb, Noël Lamandé, Sabrina Martin, Fanny Coulpier, Piotr Topilko, Isabelle Brunet Myelinating Schwann cells and Netrin-1 control intra-nervous vascularization of the developing mouse sciatic nerve eLife 2022-01-12 [PMID: 35019839]
T Hadi, L Boytard, M Silvestro, D Alebrahim, S Jacob, J Feinstein, K Barone, W Spiro, S Hutchison, R Simon, D Rateri, F Pinet, D Fenyo, M Adelman, KJ Moore, HK Eltzschig, A Daugherty, B Ramkhelawo Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells Nat Commun, 2018-11-27;9(1):5022. 2018-11-27 [PMID: 30479344] (Neutralization, Mouse)	Neutralization	Mouse
Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2 Circulation 2018-11-20 [PMID: 29976569]
K Yamahara, T Nakagawa, J Ito, K Kinoshita, K Omori, N Yamamoto Netrin 1 mediates protective effects exerted by insulin-like growth factor 1 on cochlear hair cells Neuropharmacology, 2017-04-01;119(0):26-39. 2017-04-01 [PMID: 28373074] (Functional Assay, Mouse)	Functional Assay	Mouse
Dan Liu, Si-Qi Xiong, Lei Shang, Xiao-feng Tian, Jing Yang, Xiao-Bo Xia Expression of netrin-1 receptors in retina of oxygen-induced retinopathy in mice BMC Ophthalmology 2014-12-01 [PMID: 25149138]
Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nat Med, 2014-03-02;20(4):377-84. 2014-03-02 [PMID: 24584118] (Western Blot, Mouse)	Western Blot	Mouse
van Gils , Janine M, Ramkhelawon , Bhama, Fernandes , Luciana, Stewart , Merran C, Guo , Liang, Seibert , Tara, Menezes , Gustavo, Cara , Denise C, Chow , Camille, Kinane , T Bernar, Fisher , Edward A, Balcells , Mercedes, Alvarez-Leite , Jacqueli, Lacy-Hulbert , Adam, Moore , Kathryn Endothelial expression of guidance cues in vessel wall homeostasis dysregulation under proatherosclerotic conditions. Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):911-9. 2013-02-21 [PMID: 23430612] (Neutralization, Human)	Neutralization	Human
Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J, 2013-01-08;32(5):629-44. 2013-01-08 [PMID: 23299940] (ICC, Mouse)	ICC	Mouse
Konig K, Kohler D, Granja T, Jennewein C, Tran N, Mirakaj V, Krohnert F, Rosenberger P The uncoordinated-5 homolog B receptor affects hepatic ischemia reperfusion injury. PLoS ONE, 2012-07-25;7(7):e41085. 2012-07-25 [PMID: 22848430] (Neutralization, Mouse)	Neutralization	Mouse
Janine M van Gils, Merran C Derby, Luciana R Fernandes, Bhama Ramkhelawon, Tathagat D Ray, Katey J Rayner et al. The neuroimmune guidance cue netrin-1 promotes atherosclerosis by inhibiting the emigration of macrophages from plaques Nature Immunology 2012-02-01 [PMID: 22231519]
Tadagavadi RK, Wang W Netrin-1 Regulates Th1/Th2/Th17 Cytokine Production and Inflammation through UNC5B Receptor and Protects Kidney against Ischemia-Reperfusion Injury. J. Immunol., 2010-08-06;185(0):3750-8. 2010-08-06 [PMID: 20693423] (Neutralization, Mouse)	Neutralization	Mouse
Wang W, Reeves WB, Pays L, Mehlen P, Ramesh G Netrin-1 overexpression protects kidney from ischemia reperfusion injury by suppressing apoptosis. Am. J. Pathol., 2009-08-21;175(3):1010-8. 2009-08-21 [PMID: 19700747] (IHC-P, Mouse)	IHC-P	Mouse
Hoang S, Liauw J, Choi M Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J. Cereb. Blood Flow Metab., 2008-11-05;29(2):385-97. 2008-11-05 [PMID: 18985053] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Ly NP, Komatsuzaki K, Fraser IP, Tseng AA, Prodhan P, Moore KJ, Kinane TB Netrin-1 inhibits leukocyte migration in vitro and in vivo. Proc. Natl. Acad. Sci. U.S.A., 2005-10-03;102(41):14729-34. 2005-10-03 [PMID: 16203981] (Neutralization, Human)	Neutralization	Human
Show All 16 Publications. Collapse Publications.

Array AF1006

• UNC5H2/UNC5B Antibodies
• UNC5H2/UNC5B Protein
• UNC5H2/UNC5B Proteins and Enzymes