Recombinant Mouse Slit2 (aa 26-900) Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Slit Homolog 2 (Slit2) is a member of the Slit family of secreted extracellular matrix glycoproteins that are best known for their role in axon guidance (1). It is expressed in the developing and adult brain and spinal cord, as well as in fetal lung and kidney, and the adult adrenal gland, thyroid gland, and trachea (1-3). Slit2 is composed of multiple domains including nine EGF-like domains, 20 Leucine-rich repeats (LRRs), one Laminin G-like domain, one C-terminal cysteine knot-like (CTCK) domain, and 4 N-terminal and 4 C-terminal LRR domains (1, 3). Slit2 has a molecular weight of approximately 200 kDa (4). However, proteolytic cleavage between the fifth and sixth EGF-like domains produces a membrane-bound 140 kDa N-terminal protein, termed Slit2-N, and a 55-60 kDa C-terminal fragment, termed Slit2-C (4, 5). Mature mouse Slit2 shares 96% and 98% amino acid sequence identity with the human and rat orthologs, respectively.

Slit2 has been shown to bind to multiple receptors including ROBO-1, -2, -3, and -4, Laminin-1, DAN, Gremlin, and Glypican-1 (1, 6-8). Depending upon the target, Slit2 can promote a number of diverse effects. Slit2 regulates axon guidance by binding to ROBO receptors and initiating axon repulsion (1, 5, 9-12). Slit2 has also been shown to induce growth cone collapse, inhibit oligodendrocyte precursor cell migration, and promote axon elongation, branch formation, and fasciculation (5, 13-16). Additionally, Slit2-N and Slit2-C have been shown to have distinct activities. Slit2-N binds to ROBO-1 and repels motor axon migration, while Slit2-C binds to
Glypican-1 and promotes motor axon migration (5). Outside the nervous system, Slit2 plays a role in a wide range of biological processes including cell adhesion and migration, tumor progression and metastasis, angiogenesis, lymphangiogenesis, HIV-1 replication, platelet function and thrombus formation, and stem cell senescence (1, 8, 17-30).

We have publications tested in 3 confirmed species: Human, Mouse, Xenopus.

We have publications tested in 5 applications: Adhesion Assay, Bioassay, Cell Culture, Flow Cytometry, In Vivo.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using 5444-SL	Applications	Species
Hoving, JJA;Harford-Wright, E;Wingfield-Digby, P;Cattin, AL;Campana, M;Power, A;Morgan, T;Torchiaro, E;Quereda, V;Lloyd, AC; N-cadherin directs the collective Schwann cell migration required for nerve regeneration through Slit2/3-mediated contact inhibition of locomotion eLife 2024-04-09 [PMID: 38591541] (Bioassay, Mouse)	Bioassay	Mouse
G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis Nat Commun, 2019-05-28;10(1):2350. 2019-05-28 [PMID: 31138815] (Cell Culture, Human)	Cell Culture	Human
D Pilling, LE Chinea, KM Consalvo, RH Gomer Different Isoforms of the Neuronal Guidance Molecule Slit2 Directly Cause Chemoattraction or Chemorepulsion of Human Neutrophils J. Immunol., 2018-12-03;0(0):. 2018-12-03 [PMID: 30510066] (Bioassay, Mouse)	Bioassay	Mouse
S Escot, D Willnow, H Naumann, S Di Frances, FM Spagnoli Robo signalling controls pancreatic progenitor identity by regulating Tead transcription factors Nat Commun, 2018-11-30;9(1):5082. 2018-11-30 [PMID: 30504829] (Bioassay, Human)	Bioassay	Human
N Aleksandro, I Gutsche, E Kandiah, SV Avilov, MV Petoukhov, E Seiradake, AA McCarthy Robo1 Forms a Compact Dimer-of-Dimers Assembly Structure, 2018-01-04;0(0):. 2018-01-04 [PMID: 29307485] (Human)		Human
B Erdogan, GM Cammarata, EJ Lee, BC Pratt, AF Francl, EL Rutherford, LA Lowery The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development Neural Dev, 2017-02-15;12(1):3. 2017-02-15 [PMID: 28202041] (Bioassay, Xenopus)	Bioassay	Xenopus
The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization Nat Commun, 2016-11-24;7(0):13517. 2016-11-24 [PMID: 27882935] (Bioassay, Human)	Bioassay	Human
Pilling D, Zheng Z, Vakil V, Gomer R Fibroblasts secrete Slit2 to inhibit fibrocyte differentiation and fibrosis. Proc Natl Acad Sci U S A, 2014-12-08;111(51):18291-6. 2014-12-08 [PMID: 25489114] (Bioassay, In Vivo, Human, Mouse)	Bioassay, In Vivo	Human, Mouse
Yeh M, Gonda Y, Mommersteeg M, Barber M, Ypsilanti A, Hanashima C, Parnavelas J, Andrews W Robo1 modulates proliferation and neurogenesis in the developing neocortex. J Neurosci, 2014-04-16;34(16):5717-31. 2014-04-16 [PMID: 24741061] (Bioassay, Mouse)	Bioassay	Mouse
Guijarro-Munoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis. Exp. Cell Res., 2011-12-14;318(4):371-8. 2011-12-14 [PMID: 22198087] (Adhesion Assay, Bioassay, Flow Cytometry, Human)	Adhesion Assay, Bioassay, Flow Cytometry	Human
Mulik S, Sharma S, Suryawanshi A Activation of endothelial roundabout receptor 4 reduces the severity of virus-induced keratitis. J. Immunol., 2011-05-13;186(12):7195-204. 2011-05-13 [PMID: 21572022] (In Vivo, Mouse)	In Vivo	Mouse
Brantley-Sieders DM, Dunaway CM, Rao M Angiocrine factors modulate tumor proliferation and motility through EphA2 repression of Slit2 tumor suppressor function in endothelium. Cancer Res., 2010-12-08;71(3):976-87. 2010-12-08 [PMID: 21148069] (In Vivo, Mouse)	In Vivo	Mouse
Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2010-12-06;31(3):404-16. 2010-12-06 [PMID: 21135133] (In Vivo, Mouse)	In Vivo	Mouse
Show All 13 Publications. Collapse Publications.

Array 5444-SL

• Slit2 Antibodies
• Slit2 ELISA Kits
• Slit2 Lysate
• Slit2 Proteins
• Slit2 Proteins and Enzymes