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Recombinant Human LRRC24 His-tag Protein, CF

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2 μg/lane of Recombinant Human LRRC24 His-tag (Catalog # 10512-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-55 kDa.
2 μg/lane of Recombinant Human LRRC24 His-tag (Catalog # 10512-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-55 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human LRRC24 His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human LRRC24 His-tag, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Source
Chinese Hamster Ovary cell line, CHO-derived human LRRC24 protein
Cys23-Ser365, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Cys23
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
45-55 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRRC24 His-tag Protein, CF

  • leucine rich repeat containing 24
  • leucine-rich repeat-containing protein 24
  • LRRC14OS
  • LRRC24
  • MGC111484

Background

Leucine-rich repeat-containing protein 24 (LRRC24) is type I transmembrane protein found within the large LRR superfamily. The LRR family is known to have diverse functions, including roles in innate immunity and nervous system development (1). The extracellular domain (ECD) of LRRC24 contains several LRR repeats along with an Ig-like C2-type region. The mature ECD of human LRRC24 shares 86% and 80% amino acid sequence identity with mouse and rat LRRC24, respectively. LRRC24 has been shown to interact with Robo2, which is important for axon guidance and cell migration (2). LRRC24 has been suggested to be a novel negative regulator of the ErbB family and suppress ErbB-driven tumor cell proliferation and motility (3).
  1. Ng, A.C.Y. et al. (2011) PNAS 108:4631.
  2. Söllner, C. and Wright, G.J. (2009) Genome Biol. 10:R99.
  3. Andrews, A. et al. (2017) DOI:10.21767/2254-6081-C1-003.

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Recombinant Human
LRRC24 His-tag Protei ...
10512-LR
Recombinant Human LRRC24 His-tag Protein, CF
Species: Hu
Applications: Bioactivity

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