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Recombinant Human HMGN1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human HMGN1 Protein, CF Summary

Details of Functionality
Measured by its ability to induce IL-6 secretion by monocyte-derived dendritic cells. The ED50 for this effect is 0.6-3 μg/mL
Source
E. coli-derived human HMGN1 protein
Pro2-Asp100
Accession #
N-terminal Sequence
Pro2
Protein/Peptide Type
Recombinant Proteins
Gene
HMGN1
Purity
>95%, by SDS-PAGE with silver staining
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20 kDa, reducing conditions
Publications
Read Publications using
8187-HM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human HMGN1 Protein, CF

  • FLJ31471
  • High mobility group nucleosome-binding domain-containing protein 1
  • high-mobility group (nonhistone chromosomal) protein 14
  • high-mobility group nucleosome binding 1
  • high-mobility group nucleosome binding domain 1
  • HMG14
  • HMG14FLJ27265
  • HMGN1
  • MGC104230
  • MGC117425
  • non-histone chromosomal protein HMG-14
  • nonhistone chromosomal protein HMG-14

Background

High-mobility group nucleosome-binding protein 1 (HMGN1), also known as HMG-14, is a member of the family of non-histone chromosomal proteins that play important roles in chromatin remodeling (1, 2). HMGN1 contains an N-terminal domain that interacts with nucleosomes and a C-terminal domain that assists chromatin unfolding (2, 3). Human HMGN1 shares 80% amino acid sequence identity with mouse and rat HMGN1. Alternative RNA processing generates a long isoform with a 45 amino acid insertion following Met1 (4). The trafficking of HMGN1 between the nucleus and cytoplasm is regulated by its serine phosphorylation at multiple sites (5, 6). In the nucleus, HMGN1 preferentially associates with sites of chromatin remodeling and gene transcription, allowing it to modulate the expression of a wide variety of genes (7, 8). It contributes to DNA damage repair by directly associating with and activating the double strand break sensor PARP-1 (9). HMGN1 is additionally one of several alarmins, proteins that are normally intracellular but are released following tissue damage or necrosis (1). In this setting, HMGN1 promotes dendritic cell maturation, recruitment to sites of inflammation, and production of Th1 inflammatory cytokines (10). These pro-inflammatory activities are dependent on the direct association of HMGN1 with the TLR4-MD2 complex on dendritic cells (10, 11).
  1. Yanai, H. et al. (2012) Trends Immunol. 33:633.
  2. Gerlitz, G. (2010) Biochim. Biophys. Acta 1799:80.
  3. Landsman, D. et al. (1986) J. Biol. Chem. 261:16082.
  4. Zougman, A. et al. (2008) Curr. Biol. 18:1760.
  5. Louie, D.F. et al. (2000) Protein Sci. 9:170.
  6. Pogna, E.A. et al. (2010) Biochim. Biophys. Acta 1799:93.
  7. Cuddapah, S. et al. (2011) Mol. Cell. Biol. 31:700.
  8. Kugler, J.E. et al. (2013) J. Biol. Chem. 288:16690.
  9. Masaoka, A. et al. (2012) J. Biol. Chem. 287:27648.
  10. Yang, D. et al. (2010) Biochim. Biophys. Acta 1799:157.
  11. Yang, D. et al. (2012) J. Exp. Med. 209:157.

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Publications for HMGN1/HMG14 (8187-HM)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Bioassay.


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Bioassay
(2)
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(1)
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(1)
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Bioinformatics

Gene Symbol HMGN1
Uniprot