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Recombinant Human His6-NEDD4 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human His6-NEDD4 Protein, CF Summary

Details of Functionality
Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1)&nbsp; enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.&nbsp; Reaction conditions will need to be optimized for each specific application.<br /><br />
Accession #
Protein/Peptide Type
Recombinant Enzymes

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
88 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
E3-290 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Supplied as a solution in HEPES, NaCl, DTT and EDTA.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-NEDD4 Protein, CF

  • Cell proliferation-inducing gene 53 protein
  • E3 ubiquitin-protein ligase NEDD4
  • EC 6.3.2
  • EC 6.3.2.-
  • KIAA0093NEDD4-1MGC176705
  • NEDD4
  • NEDD-4
  • Neural precursor cell expressed developmentally down-regulated protein 4
  • neural precursor cell expressed, developmentally down-regulated 4
  • receptor-potentiating factor 1
  • RPF1

Background

NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a Ubiquitin E3 ligase and the prototypical member of an extended family of HECT domain ligases.  Human family members include NEDD4, NEDD4-2, ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDDL1 and NEDDL2.  In addition to the HECT domain, NEDD4 family members also contain a C2 domain and one or more WW domains.  With a predicted molecular weight of 150 kDa, NEDD4 is expressed in most human tissues where it localizes primarily to the perinuclear and other membrane regions.  Evidence suggests that NEDD4 down-regulates multiple membrane proteins (including FGFR1, IGFR1, and multiple EGFR family members) in a Ubiquitin-dependent internalization and lysosomal degradation process.  NEDD4 also plays a role in facilitating the sequestration of alpha-synuclein to cellular endosomes.  
  1. Harvey, K.F. and S. Kumar (1999) Trends Cell Biol. 9:166.
  2. Maspero, E., et al. (2013) Nat. Struct. Mol. Biol. 20:696.
  3. Scheffner, M. and S. Kumar (2014) Biochim. Biophys. Acta. 1843:61.
  4. Sugeno, N., et al. (2014) J. Biol. Chem. 289:18137.
  5. Yang, B. and S. Kumar (2010) Cell Death Differ. 17:68.

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Publications for NEDD4 (E3-290)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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