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Recombinant Human His6-E6AP/UBE3A Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Format
Carrier-Free

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Recombinant Human His6-E6AP/UBE3A Protein, CF Summary

Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A protein
Met1 - Leu875 with a C-terminal 6-His tag
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE3A
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
102 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in HEPES, NaCl and TCEP.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-E6AP/UBE3A Protein, CF

  • ANCR
  • ANCREPVE6AP
  • AS
  • CTCL tumor antigen se37-2
  • E6AP ubiquitin-protein ligase
  • E6AP
  • E6-AP
  • EC 6.3.2.-
  • EPVE6AP
  • FLJ26981
  • HPVE6A
  • human papilloma virus E6-associated protein
  • Human papillomavirus E6-associated protein
  • Oncogenic protein-associated protein E6-AP
  • Renal carcinoma antigen NY-REN-54
  • UBE3A
  • ubiquitin protein ligase E3A
  • ubiquitin-protein ligase E3A

Background

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).
  1. Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
  2. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
  3. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
  4. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
  5. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
  6. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

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Recombinant Human
His6-E6AP/UBE3A Prote ...
E3-230
Recombinant Human His6-E6AP/UBE3A Protein, CF
Species: Hu

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Bioinformatics

Gene Symbol UBE3A
Uniprot