Recombinant Human EphA3 Fc Chimera Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

EphA3, also known as Cek4, Mek4, Hek, Tyro4, and Hek4, is a 135 kDa glycosylated member of the transmembrane Eph receptor tyrosine kinase family. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. EphA3 preferentially binds to Ephrin-A5. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). The 521 amino acid (aa) extracellular domain (ECD) of human EphA3 contains an N-terminal Ephrin binding region, a cysteine-rich region, and two fibronectin type II domains. The 418 aa cytoplasmic domain contains the tyrosine kinase domain and a sterile alpha motif (SAM) (3, 4). Within the ECD, human EphA3 shares 96% aa sequence identity with mouse and rat EphA3. Alternate splicing generates a secreted isoform that consists of nearly the entire ECD. EphA3 is expressed in the developing forebrain, retinal axons, some spinal cord motor neurons, and the heart where it plays an important role in axonal repulsion and organ morphogenesis (5 - 8). It is upregulated on some hematopoietic and solid tumor cells and on astrocytes surrounding injured nervous tissue (3, 5, 9 - 11). EphA3 ligation inhibits cellular adhesion to fibronectin as well as cellular migration (9, 10). Transmembrane EphA3 associates in cis with ADAM10 which then promotes the cleavage in trans of Ephrin-A5 (12). It also associates in cis with Ephrin-A5 on retinal axons, thereby preventing the activation of EphA3 by Ephrin-A (13). Multiple tyrosine residues within the cytoplasmic region of EphA3 become phosphorylated during ligand-induced signaling (14, 15).

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: Bioassay, ELISA Capture.

Showing Publications 1 - 3 of 3.

Publications using 6444-A3	Applications	Species
JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-03-10;0(0):101817. 2022-03-10 [PMID: 35278434] (ELISA Capture, N/A)	ELISA Capture	N/A
T Itoh, R Hatano, Y Horimoto, T Yamada, D Song, H Otsuka, Y Shirakawa, S Mastuoka, N Iwao, TM Aune, NH Dang, Y Kaneko, K Okumura, C Morimoto, K Ohnuma IL-26 mediates epidermal growth factor receptor-tyrosine kinase inhibitor resistance through endoplasmic reticulum stress signaling pathway in triple-negative breast cancer cells Cell Death & Disease, 2021-05-21;12(6):520. 2021-05-21 [PMID: 34021125] (Bioassay, Human)	Bioassay	Human
The extracellular interactome of the human adenovirus family reveals diverse strategies for immunomodulation Nat Commun, 2016-05-05;7(0):11473. 2016-05-05 [PMID: 27145901] (Human)		Human

Array 6444-A3

• EphA3 Antibodies
• EphA3 ELISA Kits
• EphA3 Lysate
• EphA3 Protein
• EphA3 Proteins and Enzymes