Recombinant Human DPPII/QPP/DPP7 Protein, CF

• 6 months from date of receipt, -20 to -70 °C as supplied.
• 3 months, -20 to -70 °C under sterile conditions after opening.

• Assay Buffer:  25 mM MES, pH 6.0
• Recombinant Human DPPII/QPP/DPP7 (rhDPP7) (Catalog # 3438-SE)
• Substrate Lys-Pro-AMC (Bachem, Catalog # I-1745), 10 mM stock in DMSO
• F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
• Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent

1. Dilute rhDPP7 to 0.2 ng/µL in Assay Buffer.
2. Dilute Substrate to 200 µM in Assay Buffer.
3. Load into a black microplate 50 µL of 0.2 ng/µL rhDPP7, and start the reaction by adding 50 μL of 200 µM Substrate. Include a Substrate Blank containing Assay Buffer in place of rhDPP7 and Substrate.
4. Read at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
5. Calculate specific activity:

     *Adjusted for Substrate Blank
     **Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891)

• rhDPP7: 0.01 µg
• Substrate: 100 µM