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Recombinant Human CD79B Protein, CF

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2 μg/lane of Recombinant Human CD79B was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 21 - 44 kDa.
2 μg/lane of Recombinant Human CD79B was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 21 - 44 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Format
Carrier-Free

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Recombinant Human CD79B Protein, CF Summary

Details of Functionality
Bioassay data are not available.
Source
Chinese Hamster Ovary cell line, CHO-derived human CD79B protein
Ala29-Asp159, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala29
Protein/Peptide Type
Innovator Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity not tested
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
21 - 44 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD79B Protein, CF

  • B29
  • B29B-cell-specific glycoprotein B29
  • B-cell antigen receptor complex-associated protein beta chain
  • CD79B antigen (immunoglobulin-associated beta)
  • CD79b antigen
  • CD79b molecule, immunoglobulin-associated beta
  • CD79B
  • IGB
  • IGBAGM6
  • Ig-beta
  • Immunoglobulin-associated B29 protein

Background

CD79B (also known as B29, Ig beta and B cell antigen receptor complex-associated protein beta-chain) is a 36-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79A. This complex interacts non-covalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79AB initiates signaling (1). Mature human CD79B is a 201 amino acid (aa) type I transmembrane glycoprotein (aa 29‑229). It contains an extracellular region with one V-type Ig-like domain (aa 38-138) and an ITAM-containing cytoplasmic domain (aa 185-213) (2). There is an alternative splice form that shows a deletion of aa 41-144 and appears after B cell activation (3). Human CD79A and B share only 26% aa identity. Over aa 29-159, human CD79B shares 54% aa identity with mouse CD79B.
  1. Tseng, J. et al. (1997). Blood 89:1513.
  2. Radaev, S, et al. (2010) Structure 18:934.
  3. Hashimoto, S. et al. (1995) Mol. Immunol. 32:651.

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