Home » B7-H7/HHLA2 » HHLA2 Peptides and Proteins » Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF

 Submit a Review

Images

 
(
Enlarge)
Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) inhibits anti-CD3 antibody induced IL-2 secretionin human T lymphocytes. The ED50 for this effect is 0.075‑0.75 μg/mL in the presence of Goat Anti-Human IgG Fc Polyclonal Antibody (Catalog # G-102-C).
Recombinant Human B7-H7/HHLA2 Fc Chimera (Catalog # 8084-B7) inhibits anti-CD3 antibody induced IL-2 secretionin human T lymphocytes. The ED50 for this effect is 0.075‑0.75 μg/mL in the presence of Goat ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.075-0.75 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human B7-H7/HHLA2 protein
Human B7-H7/HHLA2
(Ile23-Asn344)
Accession # Q9UM44		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
HHLA2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
64 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
90-100 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF

  • B7H7
  • B7-H7
  • HERV-H LTR-associating 2
  • HERV-H LTR-associating protein 2
  • HHLA2
  • Human endogenous retrovirus-H long terminal repeat-associating protein 2

Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma , TNF-alpha , IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

Customers Who Viewed This Item Also Viewed...

NKp30/NCR3
[Unconjugated]
1849-NK
NKp30/NCR3 [Unconjugated]
(19 Publications)
Species: Hu
Applications: BA
beta
2-Microglobulin Antib ...
NB500-317
Western Blot beta 2-Microglobulin Antibody (B2M-01) - BSA Free
Flow Cytometry beta 2-Microglobulin Antibody (B2M-01) - BSA Free
ELISA beta 2-Microglobulin Antibody (B2M-01) - BSA Free
(1 Review)
(3 Publications)
Species: Bv(-), Ca(-), Ch(-), Hu, Mu(-), Rb(-)
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA, WB
B7-1/CD80 Antibody
(37711) [Unconjugated ...
MAB140
Flow Cytometry B7-1/CD80 Antibody (37711) [Unconjugated]
Immunohistochemistry B7-1/CD80 Antibody (37711) [Unconjugated]
Dual RNAscope ISH-IHC Compatible B7-1/CD80 Antibody (37711) [Unconjugated]
(26 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut
MHC Class I
Antibody (OX18) - BSA
NB120-6405
Flow Cytometry MHC Class I Antibody (OX18) - BSA Free
Immunocytochemistry/ Immunofluorescence MHC Class I Antibody (OX18) - BSA Free
Immunocytochemistry/ Immunofluorescence MHC Class I Antibody (OX18) - BSA Free
(36 Publications)
Species: Rt
Applications: B/N, CyTOF-ready, EM, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
B7-H6 Antibody
(875001) [Unconjugate
MAB7144
ELISA B7-H6 Antibody (875001) [Unconjugated]
Knockout Validated B7-H6 Antibody (875001) [Unconjugated]
Flow Cytometry B7-H6 Antibody (875001) [Unconjugated]
(7 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, KO
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Flow Cytometry Pancreatic Polypeptide/PP Antibody
Immunohistochemistry-Paraffin Pancreatic Polypeptide/PP Antibody
Immunocytochemistry/ Immunofluorescence Pancreatic Polypeptide/PP Antibody
(3 Reviews)
(19 Publications)
Species: Hu, Pm, Mu, Rt
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
HHLA3 Antibody
NBP1-86331
Immunohistochemistry-Paraffin HHLA3 Antibody
Immunocytochemistry/ Immunofluorescence HHLA3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Purine Nucleoside
Phosphorylase/PNP Ant ...
NBP1-82541
Western Blot Purine Nucleoside Phosphorylase/PNP Antibody
Immunohistochemistry-Paraffin Purine Nucleoside Phosphorylase/PNP Antibody
Immunohistochemistry-Paraffin Purine Nucleoside Phosphorylase/PNP Antibody
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB

Publications for B7-H7/HHLA2 (8084-B7) (0)

There are no publications for B7-H7/HHLA2 (8084-B7).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for B7-H7/HHLA2 (8084-B7) (0)

There are no reviews for B7-H7/HHLA2 (8084-B7). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for B7-H7/HHLA2 (8084-B7) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional B7-H7/HHLA2 Products

Blogs on B7-H7/HHLA2

There are no specific blogs for B7-H7/HHLA2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human B7-H7/HHLA2 Fc Chimera Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol HHLA2
Uniprot