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Recombinant Human B7-H7 Fc Biotinylated Protein, CF

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Biotinylated Recombinant Human B7-H7/HHLA2 Fc Chimera binds to Recombinant TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) with an ED50 of < 100 ng/mL.
Biotinylated Recombinant Human B7-H7/HHLA2 Fc Chimera binds to Recombinant TMIGD2/CD28H Fc Chimera (Catalog # 8316-TR) with an ED50 of < 100 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
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Carrier-Free

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Recombinant Human B7-H7 Fc Biotinylated Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human B7-H7/HHLA2 Fc Chimera binds to Recombinant Human TMIGD2/CD28H Fc Chimera (Catalog # 8316‑TR) with an ED50 of <100 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human B7-H7/HHLA2 protein
Human B7-H7/HHLA2
(Ile23-Asn344)
Accession # Q9UM44		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ile23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
64 kDa (unlabeled).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human B7-H7 Fc Biotinylated Protein, CF

  • B7H7
  • B7-H7
  • HERV-H LTR-associating 2
  • HERV-H LTR-associating protein 2
  • HHLA2
  • Human endogenous retrovirus-H long terminal repeat-associating protein 2

Background

B7-H7, also known as HHLA2 (HERV-H LTR-associating 2), is a member of the B7 family of immune regulatory proteins (1, 2). Mature human B7-H7 consists of a 322 amino acid (aa) extracellular domain (ECD) with three immunoglobulin-like domains, a 21 aa transmembrane segment, and a 49 aa cytoplasmic domain (3-5). B7‑H7 is constitutively expressed on monocytes and is up-regulated by LPS and IFN-gamma stimulation. It is expressed on LPS/IFN-gamma treated B cells but not on resting B cells (5). B7-H7 binds to cell surface determinants on resting and mature T cells, B cells, and monocytes as well as on immature and mature dendritic cells (5). Soluble B7-H7 inhibits the proliferation of activated CD4+ and CD8+ T cells and their production of IFN-gamma , TNF-alpha , IL-5, IL-10, IL-13, IL-17A, and IL-22 (5).
  1. Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
  2. Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
  3. Mager, D.L. et al. (1999) Genomics 59:255.
  4. Flajnik, M.M. et al. (2012) Immunogenetics 64:571.
  5. Zhao, R. et al. (2013) Proc. Natl. Acad. Sci. USA 110:9879.

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