Recombinant Human Activin RIIA Fc Chimera (CHO) Protein, CF Summary
| Details of Functionality |
Measured by its ability to inhibit Activin A-induced hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. Approximately 0.01-0.05 μg/mL of Recombinant Human (rh) Activin RIIA Fc Chimera will inhibit 50% of the biological response due to 3 ng/mL of rhActivin A. |
| Source |
Chinese Hamster Ovary cell line, CHO-derived human Activin RIIA protein
Human Activin RIIA
(Met1 - Pro134)
Accession # P27037 |
IEGRMD |
Human IgG1
(Pro100 - Lys330) |
| N-terminus |
|
C-terminus |
|
|
| Accession # |
|
| N-terminal Sequence |
Ala20 |
| Structure / Form |
Disulfide-linked homodimer |
| Protein/Peptide Type |
Recombinant Proteins |
| Gene |
ACVR2A |
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Endotoxin Note |
<0.01 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
| Dilutions |
|
| Theoretical MW |
40 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| SDS-PAGE |
60-67 kDa, reducing conditions |
Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
| Purity |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
| Reconstitution Instructions |
Reconstitute at 100 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Activin RIIA Fc Chimera (CHO) Protein, CF
Background
Activin proteins are involved in a wide range of biological processes including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, the regulation of reproductive physiology, inflammation, and carcinogenesis (1 ‑ 3). They function through heteromeric complexes of type I and type II serine/threonine kinase receptors (2, 4). Dimeric ligands bind to a type II receptor, such as Activin Receptor IIA (ActRIIA), which then associates with a type I receptor to initiate signal transduction (4). ActRIIA mediates the pleiotropic effects of Activins and Inhibins as well as several members of the BMP and GDF families of TGF-beta like proteins (4). Mature human ActRIIA is a 70 kDa glycoprotein that consists of a 116 amino acid (aa) extracellular domain (ECD), a 26 aa transmembrane segment, and a 352 aa cytoplasmic region that includes the kinase domain and a PDZ-binding motif (5). Within the ECD, human ActRIIA shares 98% aa sequence identity with mouse and rat ActRIIA. Signaling through ActRIIA is modulated by its interaction with RGM-B/DRAGON, Cripto, Endoglin/CD105, TGF‑ beta RIII/Betaglycan, or BAMBI (6 - 10). These interactions can enhance ligand‑induced signaling or interfere with signaling by preventing ActRIIA association with type I receptors (6 - 9). Activin‑induced responses can also be limited by the enhanced internalization of ActRIIA following its association with the cytoplasmic protein ARIP2 (11).
- Welt, C. et al. (2002) Exp. Biol. Med. 227:724.
- Chen, Y.-G. et al. (2006) Exp. Biol. Med. 231:534.
- Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
- de Caestecker, M. (2004) Cytokine Growth Factor Rev. 15:1.
- Matzuk, M.M. and A. Bradley (1992) Biochim. Biophys Acta 1130:105.
- Samad, T.A. et al. (2005) J. Biol. Chem. 280:14122.
- Gray, P.C. et al. (2003) Proc. Natl. Acad. Sci. 100:5193.
- Lewis, K.A. et al. (2000) Nature 404:411.
- Onichtchouk, D. et al. (1999) Nature 401:480.
- Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584.
- Matsuzaki, T. et al. (2002) J. Biol. Chem. 277:19008.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: Block, IHC, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: BA
Species: Hu, Mu, Rt
Applications: BA
Species: Hu
Applications: BA, BA
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut, WB
Species: Hu
Applications: CyTOF-ready, Flow, WB
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Hu, Mu
Applications: ChIP, ICC, IHC, Simple Western, WB
Species: Mu
Applications: Block, ICC, WB
Species: Hu, Mu, Rt
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: WB
Publications for Activin RIIA (340-RC2) (0)
There are no publications for Activin RIIA (340-RC2).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Activin RIIA (340-RC2) (0)
There are no reviews for Activin RIIA (340-RC2).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
FAQs for Activin RIIA (340-RC2) (0)
Additional Activin RIIA Products
Blogs on Activin RIIA
![Western Blot TGF-beta RII Antibody [Unconjugated]](https://images.novusbio.com/images2/TGFbeta_RII_AF241NA_Western_Blot_21284.jpg)
![Neutralization TGF-beta RII Antibody [Unconjugated]](https://images.novusbio.com/images2/TGF-beta_RII_AF-241-NA_Block_Neutralize_8662.jpg)
![Immunohistochemistry TGF-beta RII Antibody [Unconjugated]](https://images.novusbio.com/images2/TGF-beta_RII_AF-241-NA_Immunohistochemistry_9941.jpg)
![Activin A [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Activin A [Unconjugated]")
![N/A Follistatin [HRP]](https://images.novusbio.com/images2/Follistatin_DFN00_ELISA_97.jpg)
![N/A Follistatin [HRP]](https://images.novusbio.com/images2/DATA_Follistatin_DFN00_ELISA_681.jpg)
![N/A Follistatin [HRP]](https://images.novusbio.com/images2/DATA_Follistatin_DFN00_ELISA_682.jpg)
![Bioactivity BMP-7 [Unconjugated]](https://images.novusbio.com/images/protein/354-bp_recombinant-human-bmp-7-protein-bioactivity-7122020141940.jpg)
![Cell Culture BMP-7 [Unconjugated]](https://images.novusbio.com/images/354-bp_recombinant-human-bmp-7-protein-cell-culture-252023113257..jpg)
![SDS-PAGE BMP-6 [Unconjugated]](https://images.novusbio.com/images2/BMP-6_507-BP_374.jpg)
![Bioactivity BMP-6 [Unconjugated]](https://images.novusbio.com/images2/BMP-6_507-BP_376.jpg)
![Bioactivity BMP-2 [Unconjugated]](https://images.novusbio.com/images/protein/355-bm_recombinant-human-mouse-rat-bmp-2-protein-bioactivity-181120209742.jpg)
![SDS-PAGE GDF-8/Myostatin [Unconjugated]](https://images.novusbio.com/images2/GDF-8_788-G8_CF_114.jpg)
![Bioactivity GDF-8/Myostatin [Unconjugated]](https://images.novusbio.com/images2/GDF-8_788-G8_CF_116.jpg)
![SDS-PAGE BMP-4 [Unconjugated]](https://images.novusbio.com/images2/BMP-4_314-BP_100.jpg)
![Bioactivity BMP-4 [Unconjugated]](https://images.novusbio.com/images2/314-bp_recombinant-human-bmp-4-protein-1882021123744.jpg)
![Western Blot BMPR-IA/ALK-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/BMPR-IA_AF346_Western_Blot_9327.jpg)
![Immunohistochemistry BMPR-IA/ALK-3 Antibody [Unconjugated]](https://images.novusbio.com/images2/af346_human-bmpr-ia-alk-3-affinity-purified-polyclonal-ab-immunohistochemistry-35202018211.jpg)
![Western Blot Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Western_Blot_5347.jpg)
![Chromatin Immunoprecipitation (ChIP) Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Chromatin_Immunoprecipitation_11240.jpg)
![Immunocytochemistry Smad2/3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Smad3_AF3797_Immunocytochemistry_11829.jpg)
![Immunocytochemistry CRIPTO Antibody [Unconjugated]](https://images.novusbio.com/images2/Cripto_AF1538_Immunocytochemistry__Immunofluorescence_20121.jpg)