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RbAp46 Antibody (OTI5A4) - Azide and BSA Free

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Western Blot: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RbAp46 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Human pancreas tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Human endometrium tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Human Kidney tissue using anti-RbAp46 mouse monoclonal antibody.
Immunohistochemistry: RbAp46 Antibody (OTI5A4) - Azide and BSA Free [NBP2-73822] - Staining of paraffin-embedded Human prostate tissue using anti-RbAp46 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI5A4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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RbAp46 Antibody (OTI5A4) - Azide and BSA Free Summary

Immunogen
Full length human recombinnat protein of RBBP7(NP_002884) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RBBP7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:500
Theoretical MW
47.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RbAp46 Antibody (OTI5A4) - Azide and BSA Free

  • G1/S transition control protein-binding protein RbAp46
  • Histone acetyltransferase type B subunit 2
  • histone-binding protein RBBP7
  • MGC138867
  • Nucleosome-remodeling factor subunit RBAP46
  • RbAp46
  • RBBP-7
  • retinoblastoma binding protein 7
  • Retinoblastoma-binding protein 7MGC138868
  • Retinoblastoma-binding protein p46
  • retinoblastoma-binding protein RbAp46

Background

RBBP7 antibody, also known as RbAp46 (Retinoblastoma protein associated protein 46), is a nuclear protein that has been shown to interact directly with retinoblastoma protein and is a component of the mSin3 corepressor complex, as are the histone deacetylase proteins (HDACs). It shares about 75% sequence identity with the closely related RbAp48, and appears to be functionally related to the yeast MSI1 protein in that overexpression of RbAp46 suppresses heat shock sensitivity in a RAS pathway dependent manner. RBBP7 has also been demonstrated to be up-regulated by the Wilms' tumor suppressor gene product, WT1. In addition, cells transfected with this antibody have shown strong inhibition of growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RBBP7