![Western Blot: Pyruvate Dehydrogenase E1 beta subunit Overexpression Lysate (Adult Normal) [NBP2-11466] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Dehydrogenase E1 beta subunit.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-beta-subunit-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11466-img0001.jpg "Western Blot: Pyruvate Dehydrogenase E1 beta subunit Overexpression Lysate (Adult Normal) [NBP2-11466] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Pyruvate Dehydrogenase E1 beta subunit.")
| Reactivity | HuSpecies Glossary |
| Applications | WB |
| Description | Pyruvate Dehydrogenase E1 beta subunit Transient Overexpression LysateExpression Host: HEK293T Plasmid: RC229907 Accession#: NM_001173468 Protein Tag: C-MYC/DDK You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT. |
| Gene | PDHB |
| Dilutions |
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| Application Notes | This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading. |
| Storage | Store at -80C. Avoid freeze-thaw cycles. |
| Buffer | RIPA buffer |
![Western Blot Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free](https://images.novusbio.com/images/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0003.jpg)
![Western Blot Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free](https://images.novusbio.com/images/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0008.jpg)
![Western Blot Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody - BSA Free](https://images.novusbio.com/images/Pyruvate-Dehydrogenase-E1-alpha-subunit-[p-Ser293]-Antibody-Western-Blot-NB110-93479-img0007.jpg)
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| Gene Symbol | PDHB |
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