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PSMA4 Antibody (OTI4B8)

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Western Blot: PSMA4 Antibody (4B8) [NBP2-01608] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMA4 monoclonal antibody.
Immunohistochemistry-Paraffin: PSMA4 Antibody (4B8) [NBP2-01608] - Staining of paraffin-embedded Human prostate tissue using anti-PSMA4 mouse monoclonal antibody.
Western Blot: PSMA4 Antibody (4B8) [NBP2-01608] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: PSMA4 Antibody (4B8) [NBP2-01608] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMA4 Antibody (4B8) [NBP2-01608] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PSMA4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMA4 Antibody (4B8) [NBP2-01608] - Staining of paraffin-embedded Human endometrium tissue using anti-PSMA4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMA4 Antibody (4B8) [NBP2-01608] - Staining of paraffin-embedded Human Kidney tissue using anti-PSMA4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PSMA4 Antibody (4B8) [NBP2-01608] - Staining of paraffin-embedded Human pancreas tissue using anti-PSMA4 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, PmSpecies Glossary
Applications WB, IHC
Clone
OTI4B8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

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PSMA4 Antibody (OTI4B8) Summary

Immunogen
Full length human recombinant protein of human PSMA4(NP_002780) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PSMA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
29.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMA4 Antibody (OTI4B8)

  • HC9
  • HC9EC 3.4.25.1
  • HsT17706
  • Macropain subunit C9
  • MGC12467
  • MGC24813
  • Multicatalytic endopeptidase complex subunit C9
  • proteasome (prosome, macropain) subunit, alpha type, 4
  • Proteasome component C9
  • proteasome subunit alpha type-4
  • proteasome subunit HC9
  • Proteasome subunit L
  • PSC9
  • PSC9MGC111191
  • PSMA4

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PSMA4
Entrez