Protein Disulfide Isomerase/P4HB Antibody Summary
| Immunogen |
This antibody was developed against Protein Disulfide Isomerase/P4HB Recombinant Protein corresponding to amino acids: LPLVIEFTEQTAPKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAESFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAVRLITLEEEMTKYKPESEELTAERITEFCHRFLEGKIKPHLMSQELPEDWDKQPVKVLVGKNF |
| Marker |
Fibroblast Marker |
| Isotype |
IgG |
| Clonality |
Polyclonal |
| Host |
Rabbit |
| Gene |
P4HB |
| Purity |
Immunogen affinity purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
- Immunohistochemistry 1:2500 - 1:5000
- Immunohistochemistry-Paraffin 1:2500 - 1:5000
- Western Blot 0.04-0.4 ug/ml
|
| Application Notes |
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. |
| Control Peptide |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
PBS (pH 7.2) and 40% Glycerol |
| Preservative |
0.02% Sodium Azide |
| Purity |
Immunogen affinity purified |
Alternate Names for Protein Disulfide Isomerase/P4HB Antibody
Background
P4HB, the beta subunit of prolyl 4-hydroxylase, a highly abundant member of the PDI family. P4HB is a multifunctional enzyme, involved in hydroxylation of prolyl residues in preprocollagen, as well as continual rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Publications for Protein Disulfide Isomerase/P4HB Antibody (NBP1-84051) (0)
There are no publications for Protein Disulfide Isomerase/P4HB Antibody (NBP1-84051).
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Protein Disulfide Isomerase/P4HB Antibody (NBP1-84051). (Showing 1 - 1 of 1 FAQ).
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We purchased Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] and are wondering if it's specific for the monomeric version of the protein or the tetrameric version? We believe this antibody is staining primary cells (isolated from mice) that are not fibroblasts. Do you know if this antibody can stain primary cells that are not fibroblasts?
- Our lab has not tested this Ab with the monomeric or multimeric subunits of the protein targets. However, the immunogen for NBP1-84051 is a.a. 236 - 379 of mouse protein (Uniprot/P09103) and since it is a polyclonal antibody (multiple epitopes), there are very strong chances that it could detect multimers also. Our lab has performed ICC/IF staining with NBP1-84051 on human U-2 OS cell line and we have not tested murine primary cells or fibroblasts yet. However, we strongly believe thatthis antibody will detect P4HB in any tissues/organs, cell lines/primary cells and fibroblasts as long as they express this protein.
Secondary Antibodies
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Isotype Controls
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Research Areas for Protein Disulfide Isomerase/P4HB Antibody (NBP1-84051)
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Blogs on Protein Disulfide Isomerase/P4HB
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0013.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining in human small intestine and testis tissues.. Corresponding P4HB RNA-seq data are presented for the same tissues.")
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP1-84051-img0007.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84051-img0009.jpg "Immunocytochemistry/Immunofluorescence: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0016.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Western-Blot-NBP1-84051-img0006.jpg "Western Blot: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Analysis in human cell line HepG2.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0014.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows no cytoplasmic positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0015.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows no cytoplasmic positivity in cells in seminiferous ducts as expected.")
![Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/Protein-Disulfide-Isomerase-P4HB-Antibody-Immunohistochemistry-Paraffin-NBP1-84051-img0017.jpg "Immunohistochemistry-Paraffin: Protein Disulfide Isomerase/P4HB Antibody [NBP1-84051] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")