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Human Prostaglandin I2 Synthase - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human Prostaglandin I2 Synthase - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-38998] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.64 ng/mL (example only; lot dependent)

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Human Prostaglandin I2 Synthase - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.64 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PTGIS

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Prostaglandin I2 Synthase - Ready-To-Use ELISA Kit (Colorimetric)

  • CYP8
  • CYP8A1
  • CYP8A1PTGI
  • CYP8prostacyclin synthase
  • EC 5.3.99.4
  • MGC126858
  • MGC126860
  • PGIS
  • PGIScytochrome P450, family 8, subfamily A, polypeptide 1
  • Prostacyclin Synthase
  • prostaglandin I2 (prostacyclin) synthase
  • Prostaglandin I2 synthase
  • PTGI
  • PTGIS

Background

PTGIS encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PTGIS