![Western Blot: Properdin Antibody (10-18) [NB100-64749] - Human serum was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. In lane 1, Bio-Rad Precision Plus molecular weight marker was run. In lane 2, Mouse anti Human Factor P was used as the primary antibody. HRP conjugated Goat anti Mouse IgG (H/L) was used as the secondary antibody. As a control, HRP conjugated Goat anti Mouse IgG (H/L) was run in lane 3. Visualization was carried out using the Bio-Rad ChemiDoc Imaging System with automatic exposure.](http://images.novusbio.com/fullsize/Properdin-Antibody-10-18-Western-Blot-NB100-64749-img0001.jpg "Western Blot: Properdin Antibody (10-18) [NB100-64749] - Human serum was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. In lane 1, Bio-Rad Precision Plus molecular weight marker was run. In lane 2, Mouse anti Human Factor P was used as the primary antibody. HRP conjugated Goat anti Mouse IgG (H/L) was used as the secondary antibody. As a control, HRP conjugated Goat anti Mouse IgG (H/L) was run in lane 3. Visualization was carried out using the Bio-Rad ChemiDoc Imaging System with automatic exposure.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, Func, IHC |
| Clone | 062-27.8.1 (10-18) |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | Native purified factor P from human serum |
| Specificity | NB100-64749 recognizes human factor P, a 53 kDa glycosylated protein present in blood serum. Factor P binds to microbial surfaces and binds and stabilizes the complement component 3- and complement component 5-convertase enzyme complexes. C3b then acts as an opsonin or initiates the complement pathway again creating a positive feedback loop which acts only on the bacterial cell, leaving the host cells unharmed. Deficiency of factor P makes the host more susceptible to bacterial infections, particularly to Neisseria species and meningococcal infections. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CFP |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Reviewed Applications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Borate buffered saline |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||||
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Enlarge |
reviewed by:
Silvia Torchio |
WB | Human | 12/29/2020 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for Properdin Antibody (NB100-64749)Find related products by research area.
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")
