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Progesterone R/NR3C3 Antibody (Alpha PR6) [mFluor Violet 500 SE]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, Gp, Pm, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
Alpha PR6
Clonality
Monoclonal
Host
Mouse
Conjugate
mFluor Violet 500 SE

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB120-2765AF350
Alexa Fluor 405
NB120-2765AF405
Alexa Fluor 488
NB120-2765AF488
Alexa Fluor 532
NB120-2765AF532
Alexa Fluor 594
NB120-2765AF594
Alexa Fluor 647
NB120-2765AF647
Alexa Fluor 700
NB120-2765AF700
Alexa Fluor 750
NB120-2765AF750
Biotin
NB120-2765B
CoraFluor 1
NB120-2765CL1
DyLight 350
NB120-2765UV
DyLight 405
NB120-2765V
DyLight 488
NB120-2765G
DyLight 550
NB120-2765R
DyLight 594
NB120-2765DL594
DyLight 650
NB120-2765C
DyLight 680
NB120-2765FR
DyLight 755
NB120-2765IR
FITC
NB120-2765F
HRP
NB120-2765H
Janelia Fluor 525
NB120-2765JF525
Janelia Fluor 549
NB120-2765JF549
Janelia Fluor 585
NB120-2765JF585
Janelia Fluor 635
NB120-2765JF635
Janelia Fluor 646
NB120-2765JF646
Janelia Fluor 669
NB120-2765JF669
Unconjugated
NB120-2765
mFluor Violet 450 SE
NB120-2765MFV450
mFluor Violet 610 SE
NB120-2765MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB120-2765
BSA Free
NB120-2765-0.025mg
BSA Free
NB120-2765
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Progesterone R/NR3C3 Antibody (Alpha PR6) [mFluor Violet 500 SE] Summary

Immunogen
Progesterone receptor purified from chick oviduct cytosol.
Localization
Nuclear
Specificity
Detects the B form of the progesterone receptor (PR). This does not cross-react with estrogen receptor or glucocorticoid receptor.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
PGR
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Primate reactivity reported in scientific literature (PMID: 18980817). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Progesterone R/NR3C3 Antibody (Alpha PR6) [mFluor Violet 500 SE]

  • NR3C3
  • NR3C3PRNuclear receptor subfamily 3 group C member 3
  • PGR
  • Progesterone R
  • progesterone receptor
  • ProgesteroneR

Background

The human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Progesterone R/NR3C3 Antibody (NB120-2765MFV500). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for PR (progesterone receptor) that would work in flow cytometry. Could you recommend some choices?
    • We do not currently have any antibodies directed towards the progesterone receptor that have been validated in flow cytometry, but any of our antibodies that work in ICC should work just fine in flow as they are essentially the same application. Please see this link for our Progesterone Receptor antibodies validated in ICC.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PGR