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Profilin 2 Antibody (5D4)

Images

 
Immunohistochemistry: Profilin 2 Antibody (5D4) [NBP3-43015] - Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (Profilin 2 Antibody) at dilution of 1:400
Western Blot: Profilin 2 Antibody (5D4) [NBP3-43015] - mouse brain tissue were subjected to SDS PAGE followed by western blot with (Profilin 2 Antibody) at dilution of 1:2000

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
5D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Profilin 2 Antibody (5D4) Summary

Immunogen
profilin 2 - (Uniprot# P35080)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PFN2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:50 - 1:500
  • Western Blot 1:1000 - 1:5000
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A or G purified

Alternate Names for Profilin 2 Antibody (5D4)

  • D3S1319E
  • PFL
  • profilin 2
  • Profilin II
  • profilin-2

Background

Profilin 2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals by binding to actin (1:1 ratio) and so affecting the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Highly expressed in skeletal muscle, kidney and brain and less strongly in placenta, heart, liver and lung.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Profilin 2 Products

Research Areas for Profilin 2 Antibody (NBP3-43015)

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Blogs on Profilin 2.

Profiling the Profilin 1 Antibody
Profilin-1, or Pfn-1, is a small actin-binding protein which plays an essential role controlling the growth of microfilaments. Profilin 1 and Profilin 2 have similar biochemical properties but are expressed in different tissues. The Profilin 1 antibod...  Read full blog post.

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Bioinformatics

Gene Symbol PFN2