![Western Blot: PRMT1 Antibody [NB100-2251] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PRMT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/PRMT1-Antibody-Western-Blot-NB100-2251-img0006.jpg "Western Blot: PRMT1 Antibody [NB100-2251] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-PRMT1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
| Reactivity | Hu, Mu, Rt, XpSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residue 300 and the C-terminus (residue 361) of human Protein Arginine N-Methyltransferase 1 using the numbering given in entry NP_001527.2 (GeneID 3276). |
| Predicted Species | Rat (100%), Xenopus (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRMT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-2251 | Applications | Species |
|---|---|---|
| Fisk JC, Zurita-Lopez C, Sayegh J et al. TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei. Eukaryot Cell 2010-06-01 [PMID: 20418380] |
Secondary Antibodies |
Isotype Controls |
Research Areas for PRMT1 Antibody (NB100-2251)Find related products by research area.
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PRMT6: One Function, Many Roles Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P... Read full blog post. |
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| Gene Symbol | PRMT1 |
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![Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PRMT1 used at a dilution of 1:200 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRMT1-Antibody-Immunohistochemistry-Paraffin-NB100-2251-img0005.jpg "Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PRMT1 used at a dilution of 1:200 (1 ug/ml). Detection: DAB")
![Western Blot: PRMT1 Antibody [NB100-2251] - Detection of Human and Mouse PRMT1 on HeLa whole cell lysate using NB100-2251. PRMT1 was also immunoprecipitated using rabbit anti-PRMT1 antibody NB100-2250.](http://images.novusbio.com/fullsize/PRMT1-Antibody-Western-Blot-NB100-2251-img0003.jpg "Western Blot: PRMT1 Antibody [NB100-2251] - Detection of Human and Mouse PRMT1 on HeLa whole cell lysate using NB100-2251. PRMT1 was also immunoprecipitated using rabbit anti-PRMT1 antibody NB100-2250.")
![Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit antiPRMT1 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRMT1-Antibody-Immunohistochemistry-Paraffin-NB100-2251-img0004.jpg "Immunohistochemistry-Paraffin: PRMT1 Antibody [NB100-2251] - Section of human head and neck squamous cell carcinoma. Antibody: Affinity purified rabbit antiPRMT1 used at a dilution of 1:1000 (0.2 ug/ml). Detection: DAB")
![Immunoprecipitation: PRMT1 Antibody [NB100-2251] - Detection of human PRMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer + 0.3% SDS, 1.5% sodium deoxycholate. Antibodies: Affinity purified rabbit anti-PRMT1 antibody NB100-2251 (lot NB100-2251-2) used for IP at 3 ug per reaction. PRMT1 was also immunoprecipitated by another rabbit anti-PRMT1 antibody. For blotting immunoprecipitated PRMT1, NB100-2251 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/PRMT1-Antibody-Immunoprecipitation-NB100-2251-img0007.jpg "Immunoprecipitation: PRMT1 Antibody [NB100-2251] - Detection of human PRMT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer + 0.3% SDS, 1.5% sodium deoxycholate. Antibodies: Affinity purified rabbit anti-PRMT1 antibody NB100-2251 (lot NB100-2251-2) used for IP at 3 ug per reaction. PRMT1 was also immunoprecipitated by another rabbit anti-PRMT1 antibody. For blotting immunoprecipitated PRMT1, NB100-2251 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.")
![Western Blot CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/CBP_MAB2676_Western_Blot_6043.jpg)
![Immunocytochemistry CBP/KAT3A Antibody (257003) [Unconjugated]](https://images.novusbio.com/images2/mab2676_human-mouse-rat-cbp-mab-clone-257003-immunocytochemistry-101220208237.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")