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PRDM14 Antibody (856235)

Images

 
PRDM14 was detected in immersion fixed D3 mouse embryonic stem cells using Rat Anti-Mouse PRDM14 Monoclonal Antibody (Catalog # MAB8097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the ...read more
PRDM14 Induces the Transition of EpiLCs to ESCLCs(A) Constructs of the ROSA-Tet-off system and piggyBac Tet-on system.(B) Western blotting of PRDM14 and tubulin.(C) Scheme of the transition of ESCs to EpiLCs and of ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ICC/IF
Clone
856235
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

PRDM14 Antibody (856235) Summary

Immunogen
E. coli-derived recombinant mouse PRDM14
Met1-Glu191
Accession # E9Q3T6
Specificity
Detects mouse PRDM14 in ELISA.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
PRDM14
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
Publications
Read Publication using
MAB8097 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PRDM14 Antibody (856235)

  • MGC59730
  • PFM11
  • PR domain containing 14
  • PR domain zinc finger protein 14
  • PR domain-containing protein 14
  • PRDM14
  • PR-domain zinc finger protein 14

Background

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 243 – 360) and six zinc finger repeats (aa 390 - 558). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Within aa 1-191, mouse PRDM14 shares 46% aa sequence identity with human PRDM14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRDM14 Antibody (MAB8097)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Immunoprecipitation, Western Blot.


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Immunoprecipitation
(1)
Western Blot
(1)
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Bioinformatics

Gene Symbol PRDM14
Uniprot