Reactivity | MuSpecies Glossary |
Applications | ICC/IF |
Clone | 856235 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | E. coli-derived recombinant mouse PRDM14 Met1-Glu191 Accession # E9Q3T6 |
Specificity | Detects mouse PRDM14 in ELISA. |
Source | N/A |
Isotype | IgG2b |
Clonality | Monoclonal |
Host | Rat |
Gene | PRDM14 |
Purity Statement | Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Reconstitution Instructions | Reconstitute at 0.5 mg/mL in sterile PBS. |
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 243 – 360) and six zinc finger repeats (aa 390 - 558). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Within aa 1-191, mouse PRDM14 shares 46% aa sequence identity with human PRDM14.
Publication using MAB8097 | Applications | Species |
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PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency Stem Cell Reports, 2016-11-17;7(6):1072-1086. 2016-11-17 [PMID: 27866876] (Immunoprecipitation, Western Blot, Mouse) | Immunoprecipitation, Western Blot | Mouse |
Secondary Antibodies |
Isotype Controls |
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