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PML Protein Antibody

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Independent Antibodies: Western Blot: PML Protein Antibody [NB100-59788] - Detection of Human PML on HeLa whole cell lysate using NB100-59788. PML was also immunoprecipitated by rabbit anti-PML antibodies ...read more
Immunohistochemistry-Paraffin: PML Protein Antibody [NB100-59788] - FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PML used at a dilution of 1:200 (1ug/ml). Detection: DAB

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

       

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PML Protein Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and 625 of promyelocytic leukemia using the numbering given in entry NP_150241.2 (GeneID 5371).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PML
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using NB100-59788.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PML Protein Antibody

  • MYLPP8675
  • Promyelocytic leukemia protein
  • promyelocytic leukemia
  • RING finger protein 71
  • RNF71probable transcription factor PML
  • TRIM19promyelocytic leukemia, inducer of
  • tripartite motif protein TRIM19
  • Tripartite motif-containing protein 19

Background

PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PML Protein Antibody (NB100-59788)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PML
Entrez
Uniprot