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PLC-gamma 1 Antibody (479819)

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC- gamma1 Monoclonal Antibody (Catalog # MAB32881) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLC- gamma1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue. PVDF membrane was ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clone
479819
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB32881-SP
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PLC-gamma 1 Antibody (479819) Summary

Immunogen
E. coli-derived recombinant human PLC-gamma 1
Phe550-His663
Accession # P19174
Specificity
Detects endogenous human, mouse, and rat PLC-gamma 1.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
PLCG1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PLC-gamma 1 Antibody (479819)

  • EC 3.1.4.11
  • gamma 1 (formerly subtype 148)
  • monophosphatidylinositol phosphodiesterase
  • NCKAP3,1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1
  • phosphatidylinositol phospholipase C
  • phosphoinositidase C
  • phosphoinositide phospholipase C
  • Phosphoinositide phospholipase C-gamma-1
  • phospholipase C, gamma 1
  • phospholipase C-148
  • Phospholipase C-gamma-1
  • Phospholipase C-II
  • PLC11-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1
  • PLC148
  • PLC-148
  • PLCG1
  • PLCgamma 1
  • PLC-gamma 1
  • PLC-gamma-1
  • PLC-II1-phosphatidyl-D-myo-inositol-4,5-bisphosphate
  • PPLCA
  • triphosphoinositide phosphodiesterase

Background

PLC-gamma 1 (phospholipase C-gamma 1) cleaves the polar head group from the membrane lipid phosphatidyl-inositol 4,5-biphosphate. The consequence of this reaction is the generation of two secondary messengers, inositol 1,4,5-trisphosphate and diacylglycerol, which mediate the mobilization of intracellular calcium and the activation of protein kinase C, respectively. Members of the PLC family are regulated by different signaling pathways. PLC-gamma 1 is regulated through receptor and non-receptor tyrosine kinases, while the PLC-beta isoforms are regulated by G-protein coupled receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol PLCG1
Uniprot