PLC-delta 3 Antibody Summary
Immunogen |
Peptide with sequence C-HQYSGEDRVLSAPE, from the internal region of the protein sequence according to NP_588614.1. |
Epitope |
aa261-274 |
Predicted Species |
Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Goat |
Gene |
PLCD3 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry
- Immunohistochemistry-Paraffin 6 ug/ml
- Peptide ELISA Detection limit 1:32000
- Western Blot 1-3 ug/ml
|
Application Notes |
Western blot: Approx 85kDa band observed in lysates of cell limes A549 and Caco-2, and in preliminary testing of cell lines HeLa, HEK293 and U251 lysate (calculated MW of 89.3kDa according to NP_588614.1). Primary incubation 1 hour at room temperature. |
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
Preservative |
0.02% Sodium Azide |
Concentration |
0.5 mg/ml |
Purity |
Immunogen affinity purified |
Alternate Names for PLC-delta 3 Antibody
Background
PLCD3, also referred to as Phospholipase C-delta-3, is 789 amino acids in length and approximately 89kDa. PLCD3 is a member of the Phospholipase C family and functions to hydrolyze PIP2 to produce the second messangers DAG and IP3. Current research on PLCD3 is being conducted in relation to several diseases and disorders including breast cancer, glioblastoma, Huntington's disease and neurological diseases. PLCD3 has also been shown to have interactions with ITPR1, ITPR3, PIK3C3, DGKE and SKIL in pathways such as the CREB pathway, sweet taste signaling and Inositol phosphate metabolism.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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