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Plakophilin-3 Antibody (23E3/4) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clone
23E3/4
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

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Plakophilin-3 Antibody (23E3/4) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
This antibody is a mouse monoclonal IgG2b antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of Human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).
Specificity
This antibody reacts with an epitope located between residues 570-589 in Human plakophilin-3.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PKP3
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Plakophilin-3 Antibody (23E3/4) [CoraFluor™ 1]

  • plakophilin 3
  • plakophilin-3

Background

The Plakophilin-3 gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Plakophilin-3 Antibody (NBP1-97674CL1) (0)

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Bioinformatics

Gene Symbol PKP3