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Human PKM2 ELISA Kit (Colorimetric)

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ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Samples were spiked with high concentrations of Human PKM2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range ...read more
ELISA: Human PKM2 ELISA Kit (Colorimetric) [NBP3-18036] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63-1000 IU/mL
Sensitivity
9.38 IU/mL

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Human PKM2 ELISA Kit (Colorimetric) Summary

Standard Curve Range
15.63-1000 IU/mL
Sensitivity
9.38 IU/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 5.23
Intra-Assay
%CV < 5.67
Spike Recovery
88-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PKM

Applications/Dilutions

Dilutions
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human PKM2 ELISA Kit (Colorimetric)

  • CTHBP
  • Cytosolic thyroid hormone-binding protein
  • MGC3932
  • OIP3
  • OIP-3
  • OIP3EC 2.7.1.40
  • Opa-interacting protein 3
  • p58
  • PK, muscle type
  • PK2
  • PK3
  • PK3PKM
  • PKM2
  • Pyruvate kinase 2/3
  • pyruvate kinase isozymes M1/M2
  • Pyruvate kinase muscle isozyme
  • pyruvate kinase, muscle
  • TCB
  • THBP1
  • THBP1p58
  • Thyroid hormone-binding protein 1
  • thyroid hormone-binding protein, cytosolic
  • Tumor M2-PK

Background

PKM2 is encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for PKM2 ELISA Kit (NBP3-18036) (0)

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MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
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5 Stars for the PKM2 Antibody
Novus' Pyruvate Kinase M2 antibody (cat # NBP1-48308) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "The best thing about this antibody is that it works well for immunofluorescence staining of fr...  Read full blog post.

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Bioinformatics

Gene Symbol PKM