![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0002.jpg "Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Bv, Rb, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clone | GT1810 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PKM2. The exact sequence is proprietary. |
| Predicted Species | Bovine (97%), Rabbit (97%), Xenopus (90%). Backed by our 100% Guarantee. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PKM |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-P-Assay dependent |
| Theoretical MW | 58 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
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Secondary Antibodies |
Isotype Controls |
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5 Stars for the PKM2 Antibody Novus' Pyruvate Kinase M2 antibody (cat # NBP1-48308) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "The best thing about this antibody is that it works well for immunofluorescence staining of fr... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PKM |
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632] - PKM2 antibody [GT1810] detects PKM2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunocytochemistry-Immunofluorescence-NBP3-13632-img0003.jpg "Immunocytochemistry/Immunofluorescence: PKM2 Antibody (GT1810) [NBP3-13632] - PKM2 antibody [GT1810] detects PKM2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.")
![Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632] - Human lung cancer. PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Immunohistochemistry-Paraffin-NBP3-13632-img0004.jpg "Immunohistochemistry-Paraffin: PKM2 Antibody (GT1810) [NBP3-13632] - Human lung cancer. PKM2 stained by PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PKM2-Antibody-GT1810-Western-Blot-NBP3-13632-img0001.jpg "Western Blot: PKM2 Antibody (GT1810) [NBP3-13632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKM2 antibody [GT1810] (NBP3-13632) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")