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PIP Antibody (PIP/1571) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, MA, CyTOF-ready
Clone
PIP/1571
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

Order Details

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PIP Antibody (PIP/1571) [Janelia Fluor® 549] Summary

Immunogen
Recombinant human PIP protein fragment (around aa 41-146) (exact sequence is proprietary) (Uniprot: P12273)
Localization
Cytoplasmic (Golgi bodies) and Secreted
Marker
Breast Marker
Specificity
It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15). It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. GCDFP-15, also known as PIP, for prolactin inducible protein, is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PIP
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for PIP Antibody (PIP/1571) [Janelia Fluor® 549]

  • GCDFP15prolactin-inducible protein
  • GCDFP-15SABP
  • GPIP4gp17
  • Gross cystic disease fluid protein 15
  • prolactin-induced proteinSecretory actin-binding protein

Background

The androgen and prolactin responsive prolactin-inducible protein (PIP), also known as Gross cystic disease fluid protein (GCDFP-15) is expressed in benign and malignant human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive PIP gene is expressed in benign and malignant breast tumor tissues and in such normal exocrine organs as sweat, salivary, and lacrimal glands. The cells within the body that produce PIP/GCDFP 15 appear to be restricted primarily to those with apocrine function. Studies have found PIP to be a highly specific and sensitive marker for breast cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PIP