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Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39341] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
3.12 - 200 U/L (example only; lot dependent)
Sensitivity
1.6 U/L (example only; lot dependent)

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Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
3.12 - 200 U/L (example only; lot dependent)
Sensitivity
1.6 U/L (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PIK3C2A

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human PIK3C2A - Ready-To-Use ELISA Kit (Colorimetric)

  • C2-containing phosphatidylinositol kinase
  • CPK
  • DKFZp686L193
  • EC 2.7.1
  • EC 2.7.1.137
  • EC 2.7.1.153
  • EC 2.7.1.154
  • MGC142218
  • phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha
  • Phosphoinositide 3-kinase-C2-alpha
  • phosphoinositide-3-kinase, class 2, alpha polypeptide
  • PI3-K-C2(ALPHA)
  • PI3-K-C2A
  • PI3K-C2alpha
  • PI3K-C2-alpha
  • PIK3C2A
  • PtdIns-3-kinase C2 subunit alpha

Background

PIK3C2A belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PIK3C2A