![Western Blot: Phospholipase C beta 2 Antibody [NBP3-13288] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Western-Blot-NBP3-13288-img0004.jpg "Western Blot: Phospholipase C beta 2 Antibody [NBP3-13288] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membranes were blotted with Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RMSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Phospholipase C beta 2. The exact sequence is proprietary. |
| Predicted Species | Rhesus Macaque (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLCB2 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | IHC-Fr Assay dependent |
| Theoretical MW | 134 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Phospholipase C beta 2 Antibody (NBP3-13288)Find related products by research area.
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| Gene Symbol | PLCB2 |
![Immunocytochemistry/Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-13288] - Phospholipase C beta 2 antibody detects Phospholipase C beta 2 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13288-img0003.jpg "Immunocytochemistry/Immunofluorescence: Phospholipase C beta 2 Antibody [NBP3-13288] - Phospholipase C beta 2 antibody detects Phospholipase C beta 2 protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288] - Human lung cancer. Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13288-img0002.jpg "Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288] - Human lung cancer. Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288] - Human colon cancer. Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Phospholipase-C-beta-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13288-img0001.jpg "Immunohistochemistry-Paraffin: Phospholipase C beta 2 Antibody [NBP3-13288] - Human colon cancer. Phospholipase C beta 2 stained by Phospholipase C beta 2 antibody (NBP3-13288) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")