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Human Phospholipase B1 - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human Phospholipase B1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39365] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates and other biological fluids
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.113 ng/mL (example only; lot dependent)

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Human Phospholipase B1 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
0.312 - 20 ng/mL (example only; lot dependent)
Sensitivity
0.113 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PLB1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Phospholipase B1 - Ready-To-Use ELISA Kit (Colorimetric)

  • FLJ30866
  • hPLB
  • phospholipase B1
  • PLBmembrane-associated

Background

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity includingphospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids anddiacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activitytoward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PLB1